Comparison of two total RNA extraction protocols from CHO-K1 cells for RT-PCR: cutoff cost for researchers

• Main Authors: Packeer Mohamed, Vasila, Hashim, Yumi Zuhanis Has-Yun, Amid, A., Mel, Maizirwan
• Format: Article
• Language: English
• Published: IIUM Press 2014
• Subjects: Q Science (General)
• Online Access: http://irep.iium.edu.my/38026/1/Paper_-_Packeer_Mohamed_2014_%28IIUM_EJ%29.pdf; http://irep.iium.edu.my/38026/; http://journals.iium.edu.my/ejournal/index.php/iiumej
• Institution: Universiti Islam Antarabangsa Malaysia

Various methods have been described to extract RNA from adherent mammalian cells. RNA isolation in conjunction with reverse transcription polymerase chain reaction (RT-PCR) is a valuable tool used to study gene expression profiling. This approach is now being used in mammalian cell bioprocessing to...