Kinetic study of acetic acid (vinegar) production from start fruit juice in a bioreactor

• Main Authors: Abdul Karim, Mohamed Ismail, Jamal, Parveen, Kamaruddin, Noor Izzaida
• Format: Article
• Language: English; English
• Published: Journal of Pure and Applied Microbiology 2014
• Subjects: TA164 Bioengineering
• Online Access: http://irep.iium.edu.my/53396/1/53396_Kinetic%20study%20of%20acetic%20acid%20%28vinegar%29%20production%20from%20start%20fruit%20juice%20in%20a%20bioreactor_Publisher%27s%20website.pdf; http://irep.iium.edu.my/53396/2/53396_Kinetic%20study%20of%20acetic%20acid%20%28vinegar%29%20production%20from%20start%20fruit%20juice%20in%20a%20bioreactor_SCOPUS.pdf; http://irep.iium.edu.my/53396/; http://www.microbiologyjournal.org/jmabsread.php?snoid=334&month=&year=
• Institution: Universiti Islam Antarabangsa Malaysia

A two stage fermentation process for production of acetic acid using star fruit juice with Saccaromycescerevisiae and Acetomonasacetii was conducted in a 2 L stirred tank bioreactor. The effect of inoculum size ( 5%,10%,15%) and agitation speed ( 250 rpm, 300 rpm, 350 rpm) on ethanol and acetic acid production of the juice were evaluated. Kinetic parameters on specific growth rate, yield, doubling time (i.e. ?,Yp/s. td,) of the fermentation process were also recorded. The optimum condition obtained from this batch fermentation was at 250 rpm of agitation speed and 5% inoculums sizes with the maximum product yield (Yethanol/glucose) of 0.859 g/g and (Y Acetic acid/Ethanol) of 0.342 g/g. The maximum specific growth rate, ? was 0.097h-1 for Saccharomyces cerevisiae while 0.085h-1 for Acetobacteraceti using initial glucose concentration of 20%. Using Central Composite Design experiment, for optimization of acetic acid production, the R2 value of 0.8372 was achieved and the highest acetic acid production obtained was 2.76 %TA while highest ethanol obtained was 17.8% or equivalent to 140.64 g/L. The above findings can be further utilized in fermentation process to produce vinegar in large amount from our tropical star fruit, Averrhoacarambola juice.