Effects of Sargassum oligocystum and Padina australis extract on Adipogenesis and Adipolysis in 3T3-L1 Cells

In recent years, the search for natural, safe and effective therapies for the management of obesity has become important. The present study investigated the potential of brown seaweeds Sargassum oligocystum and Padina australis from Malaysian waters as natural alternatives for the management of o...

Full description

Saved in:
Bibliographic Details
Main Authors: Jaswir, Irwandi, Ahmad, Hasna, Darnis, Deny Susanti, Bakhtiar, M. Taher, Octavianti, Fitri
Format: Article
Language:English
English
Published: Faculty of Food Science & Technology, Universiti Putra Malaysia (UPM) 2017
Subjects:
Online Access:http://irep.iium.edu.my/60854/1/60854_Effects%20of%20Sargassum%20oligocystum.pdf
http://irep.iium.edu.my/60854/7/60854_Effects%20of%20Sargassum%20oligocystum%20and%20Padina_new_scopus.pdf
http://irep.iium.edu.my/60854/
http://www.ifrj.upm.edu.my/24%20(07)%202017%20supplementary/(21)%20R1.pdf
Tags: Add Tag
No Tags, Be the first to tag this record!
Institution: Universiti Islam Antarabangsa Malaysia
Language: English
English
id my.iium.irep.60854
record_format dspace
spelling my.iium.irep.608542018-04-19T13:39:11Z http://irep.iium.edu.my/60854/ Effects of Sargassum oligocystum and Padina australis extract on Adipogenesis and Adipolysis in 3T3-L1 Cells Jaswir, Irwandi Ahmad, Hasna Darnis, Deny Susanti Bakhtiar, M. Taher Octavianti, Fitri QD Chemistry TP Chemical technology In recent years, the search for natural, safe and effective therapies for the management of obesity has become important. The present study investigated the potential of brown seaweeds Sargassum oligocystum and Padina australis from Malaysian waters as natural alternatives for the management of obesity. Both seaweeds were macerated using acetone at room temperature for ten hours. The S. oligocystum extracts (SE) and P. australis extracts (PE) were then applied to 3T3-L1 cells during the differentiation stage and during the mature stage of the adipocyte life cycle to assess the effects of extracts on adipogenesis and adipolysis. Application of SE at 12.5 and 50 µg/ml to the cells decreased adipogenesis by 71.7%, and 84.8%, respectively, while cells treated with 12.5 and 50 µg/ml PE showed 85.7%, and 89.0% adipogenesis respectively, compared to control. Application of SE and PE to mature lipid cells stimulated adipolysis and the release of glycerol into the culture media. Application of SE at 12.5 and 50 µg µg/ ml in the cell media induced glycerol release by up to 88.6 and 93.0%, respectively, while PE increased glycerol release up to 92.9 and 95.6% respectively, compared to isoproterenol. This study demonstrates the potential of whole brown seaweed extracts from S. oligocystum and P. australis collected from Malaysian waters as natural anti- obesity agents. Incorporation of the brown seaweed into the diet as a functional food will be useful for prevention and treatment of obesity. Faculty of Food Science & Technology, Universiti Putra Malaysia (UPM) 2017-12 Article REM application/pdf en http://irep.iium.edu.my/60854/1/60854_Effects%20of%20Sargassum%20oligocystum.pdf application/pdf en http://irep.iium.edu.my/60854/7/60854_Effects%20of%20Sargassum%20oligocystum%20and%20Padina_new_scopus.pdf Jaswir, Irwandi and Ahmad, Hasna and Darnis, Deny Susanti and Bakhtiar, M. Taher and Octavianti, Fitri (2017) Effects of Sargassum oligocystum and Padina australis extract on Adipogenesis and Adipolysis in 3T3-L1 Cells. International Food Research Journal, 24 (Suppl.). pp. 395-401. ISSN 1985-4668 http://www.ifrj.upm.edu.my/24%20(07)%202017%20supplementary/(21)%20R1.pdf
institution Universiti Islam Antarabangsa Malaysia
building IIUM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider International Islamic University Malaysia
content_source IIUM Repository (IREP)
url_provider http://irep.iium.edu.my/
language English
English
topic QD Chemistry
TP Chemical technology
spellingShingle QD Chemistry
TP Chemical technology
Jaswir, Irwandi
Ahmad, Hasna
Darnis, Deny Susanti
Bakhtiar, M. Taher
Octavianti, Fitri
Effects of Sargassum oligocystum and Padina australis extract on Adipogenesis and Adipolysis in 3T3-L1 Cells
description In recent years, the search for natural, safe and effective therapies for the management of obesity has become important. The present study investigated the potential of brown seaweeds Sargassum oligocystum and Padina australis from Malaysian waters as natural alternatives for the management of obesity. Both seaweeds were macerated using acetone at room temperature for ten hours. The S. oligocystum extracts (SE) and P. australis extracts (PE) were then applied to 3T3-L1 cells during the differentiation stage and during the mature stage of the adipocyte life cycle to assess the effects of extracts on adipogenesis and adipolysis. Application of SE at 12.5 and 50 µg/ml to the cells decreased adipogenesis by 71.7%, and 84.8%, respectively, while cells treated with 12.5 and 50 µg/ml PE showed 85.7%, and 89.0% adipogenesis respectively, compared to control. Application of SE and PE to mature lipid cells stimulated adipolysis and the release of glycerol into the culture media. Application of SE at 12.5 and 50 µg µg/ ml in the cell media induced glycerol release by up to 88.6 and 93.0%, respectively, while PE increased glycerol release up to 92.9 and 95.6% respectively, compared to isoproterenol. This study demonstrates the potential of whole brown seaweed extracts from S. oligocystum and P. australis collected from Malaysian waters as natural anti- obesity agents. Incorporation of the brown seaweed into the diet as a functional food will be useful for prevention and treatment of obesity.
format Article
author Jaswir, Irwandi
Ahmad, Hasna
Darnis, Deny Susanti
Bakhtiar, M. Taher
Octavianti, Fitri
author_facet Jaswir, Irwandi
Ahmad, Hasna
Darnis, Deny Susanti
Bakhtiar, M. Taher
Octavianti, Fitri
author_sort Jaswir, Irwandi
title Effects of Sargassum oligocystum and Padina australis extract on Adipogenesis and Adipolysis in 3T3-L1 Cells
title_short Effects of Sargassum oligocystum and Padina australis extract on Adipogenesis and Adipolysis in 3T3-L1 Cells
title_full Effects of Sargassum oligocystum and Padina australis extract on Adipogenesis and Adipolysis in 3T3-L1 Cells
title_fullStr Effects of Sargassum oligocystum and Padina australis extract on Adipogenesis and Adipolysis in 3T3-L1 Cells
title_full_unstemmed Effects of Sargassum oligocystum and Padina australis extract on Adipogenesis and Adipolysis in 3T3-L1 Cells
title_sort effects of sargassum oligocystum and padina australis extract on adipogenesis and adipolysis in 3t3-l1 cells
publisher Faculty of Food Science & Technology, Universiti Putra Malaysia (UPM)
publishDate 2017
url http://irep.iium.edu.my/60854/1/60854_Effects%20of%20Sargassum%20oligocystum.pdf
http://irep.iium.edu.my/60854/7/60854_Effects%20of%20Sargassum%20oligocystum%20and%20Padina_new_scopus.pdf
http://irep.iium.edu.my/60854/
http://www.ifrj.upm.edu.my/24%20(07)%202017%20supplementary/(21)%20R1.pdf
_version_ 1643615881588113408