Antinociceptive activity of asiaticoside in mouse models of induced nociception

• Main Authors: Ayumi, Rasyidah Ryta, Shaik Mossadeq, Wan Mastura, Zakaria, Zainul Amiruddin, Bakhtiar, M. Taher, Kamarudin, Nadhirah, Hisamuddin, Nadia, Talib, Madihah, Sabar, Aina Mardhiah
• Format: Article
• Language: English; English; English
• Published: Georg Thieme Verlag KG Stuttgart 2020
• Subjects: RS Pharmacy and materia medica; RS192 Materia Medica-Pharmaceutical Technology
• Online Access: http://irep.iium.edu.my/80138/1/Planta%20Medica.pdf; http://irep.iium.edu.my/80138/7/80138_Antinociceptive%20Activity%20of%20Asiaticoside%20in%20Mouse_SCOPUS.pdf; http://irep.iium.edu.my/80138/13/80138_Erratum%20Antinociceptive%20Activity%20of%20Asiaticoside%20in%20Mouse_SCOPUS.pdf; http://irep.iium.edu.my/80138/; https://www.thieme-connect.com/products/ejournals/abstract/10.1055/a-1144-3663
• Institution: Universiti Islam Antarabangsa Malaysia

The antinociceptive property of Centella asiatica extracts is known but the analgesic activity of its bioactive constituent asiaticoside has not been reported. We evaluated the antino- ciceptive activity of orally (p.o.) administered asiaticoside (1, 3, 5, and 10mg/kg) in mice using the 0.6% acetic acid-in- duced writhing test, the 2.5% formalin-induced paw licking test, and the hot plate test. The capsaicin- and glutamate-in- duced paw licking tests were employed to evaluate the in- volvement of the vanilloid and glutamatergic systems, respec- tively. Asiaticoside (3, 5, and 10 mg/kg, p. o.) reduced the rate of writhing (p < 0.0001) by 25.3, 47.8, and 53.9 %, respective- ly, and increased the latency period (p < 0.05) on the hot plate at 60 min post-treatment until the end of the experiment. Moreover, asiaticoside (3, 5, and 10 mg/kg, p. o.) shortened the time spent in licking/biting the injected paw (p < 0.0001) in the early phase of the formalin test by 45.7, 51.4, and 52.7 %, respectively, and in the late phase (p < 0.01) by 23.6, 40.5, and 50.6 %, respectively. Antinociception induced by asi- aticoside (10 mg/kg) was not antagonized by naloxone in both the 2.5 % formalin-induced nociception and the hot plate test, indicating a nonparticipation of the opioidergic system. Asia- ticoside (1, 3, 5, and 10 mg/kg, p. o.) reduced the duration of biting/licking the capsaicin-injected paw (p < 0.0001) by 40.5, 48.2, 59.5, and 63.5%, respectively. Moreover, asiaticoside (5 and 10 mg/kg) shortened the time spent in biting/licking the glutamate-injected paw (p < 0.01) by 29.9 and 48.6 %, re- spectively. Therefore, asiaticoside (5 and 10 mg/kg, p. o.) in- duces antinociception possibly through the vanilloid and glu- tamatergic systems.