Characterization and functionalisation of activated carbon for the enhancement of enzyme catalyst activity

Background and objective: Activated carbon is commonly used as an immobilisation matrix due to its large surface area, making it a highly desirable matrix for use in immobilising enzymes as preparation for use on the industrial scale. The objective of this research is to determine the effectiveness...

Full description

Saved in:
Bibliographic Details
Main Authors: Kabbashi, Nassereldeen, Kadouf, Yara, Bello, Ibrahim Adebayo, Mirghani, Mohamed Elwathig Saeed, Musa, Lubna
Format: Article
Language:English
Published: Elsevier 2021
Subjects:
Online Access:http://irep.iium.edu.my/91786/7/91786_Characterization%20and%20functionalisation%20of%20activated%20carbon.pdf
http://irep.iium.edu.my/91786/
https://www.journals.elsevier.com/materials-science-and-engineering-b
Tags: Add Tag
No Tags, Be the first to tag this record!
Institution: Universiti Islam Antarabangsa Malaysia
Language: English
id my.iium.irep.91786
record_format dspace
spelling my.iium.irep.917862021-08-28T10:09:03Z http://irep.iium.edu.my/91786/ Characterization and functionalisation of activated carbon for the enhancement of enzyme catalyst activity Kabbashi, Nassereldeen Kadouf, Yara Bello, Ibrahim Adebayo Mirghani, Mohamed Elwathig Saeed Musa, Lubna TP155 Chemical engineering Background and objective: Activated carbon is commonly used as an immobilisation matrix due to its large surface area, making it a highly desirable matrix for use in immobilising enzymes as preparation for use on the industrial scale. The objective of this research is to determine the effectiveness of different acids for functionalisation on immobilisation capacity and also to characterize the functionalized activated carbon for the functional groups present. Materials and methods: Activated carbon was functionalised with three acids (hydrochloric acid, nitric acid and sulphuric acid) along with a control sample washed with distilled water. Immobilisation capacity was calculated with hydrochloric acid functionalized activated carbon (HCl-FAC) giving the highest immobilization capacity (6.022 U/g). Characterisation of the functionalised activated carbon was conducted using FT-IR (Fourier Transform Infra-Red) spectroscopy analysis of the samples with the aim of analyzing the various functional groups present to determine the sample with distinct characteristics thus telling the degree of adsorption of lipase onto the activated carbon powder. Results: HNO3-FAC (functionalized activated carbon) showed a very distinct pattern as a larger number of surface functional groups emerged. The immobilisation on a matrix ensures thermal stability and increased reusability of the enzyme. Therefore, in this research, lipase sourced from Candida antarctica was immobilised on acid functionalised activated carbon. The best acid for functionalisation was found to be hydrochloric acid. Conclusion: Due to the very distinct patterns shown by the FT-IR spectrum of the HNO3-FAC after a fair comparison with others, it allows for a larger number of surface functional groups which will definitely enhance the stability of the enzyme lipase. Elsevier 2021-09 Article PeerReviewed application/pdf en http://irep.iium.edu.my/91786/7/91786_Characterization%20and%20functionalisation%20of%20activated%20carbon.pdf Kabbashi, Nassereldeen and Kadouf, Yara and Bello, Ibrahim Adebayo and Mirghani, Mohamed Elwathig Saeed and Musa, Lubna (2021) Characterization and functionalisation of activated carbon for the enhancement of enzyme catalyst activity. Journal of Materials Science and Engineering B, 11 (4-6). pp. 81-87. ISSN 0921-5107 https://www.journals.elsevier.com/materials-science-and-engineering-b 10.17265/2161-6221/2021.4-6.005
institution Universiti Islam Antarabangsa Malaysia
building IIUM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider International Islamic University Malaysia
content_source IIUM Repository (IREP)
url_provider http://irep.iium.edu.my/
language English
topic TP155 Chemical engineering
spellingShingle TP155 Chemical engineering
Kabbashi, Nassereldeen
Kadouf, Yara
Bello, Ibrahim Adebayo
Mirghani, Mohamed Elwathig Saeed
Musa, Lubna
Characterization and functionalisation of activated carbon for the enhancement of enzyme catalyst activity
description Background and objective: Activated carbon is commonly used as an immobilisation matrix due to its large surface area, making it a highly desirable matrix for use in immobilising enzymes as preparation for use on the industrial scale. The objective of this research is to determine the effectiveness of different acids for functionalisation on immobilisation capacity and also to characterize the functionalized activated carbon for the functional groups present. Materials and methods: Activated carbon was functionalised with three acids (hydrochloric acid, nitric acid and sulphuric acid) along with a control sample washed with distilled water. Immobilisation capacity was calculated with hydrochloric acid functionalized activated carbon (HCl-FAC) giving the highest immobilization capacity (6.022 U/g). Characterisation of the functionalised activated carbon was conducted using FT-IR (Fourier Transform Infra-Red) spectroscopy analysis of the samples with the aim of analyzing the various functional groups present to determine the sample with distinct characteristics thus telling the degree of adsorption of lipase onto the activated carbon powder. Results: HNO3-FAC (functionalized activated carbon) showed a very distinct pattern as a larger number of surface functional groups emerged. The immobilisation on a matrix ensures thermal stability and increased reusability of the enzyme. Therefore, in this research, lipase sourced from Candida antarctica was immobilised on acid functionalised activated carbon. The best acid for functionalisation was found to be hydrochloric acid. Conclusion: Due to the very distinct patterns shown by the FT-IR spectrum of the HNO3-FAC after a fair comparison with others, it allows for a larger number of surface functional groups which will definitely enhance the stability of the enzyme lipase.
format Article
author Kabbashi, Nassereldeen
Kadouf, Yara
Bello, Ibrahim Adebayo
Mirghani, Mohamed Elwathig Saeed
Musa, Lubna
author_facet Kabbashi, Nassereldeen
Kadouf, Yara
Bello, Ibrahim Adebayo
Mirghani, Mohamed Elwathig Saeed
Musa, Lubna
author_sort Kabbashi, Nassereldeen
title Characterization and functionalisation of activated carbon for the enhancement of enzyme catalyst activity
title_short Characterization and functionalisation of activated carbon for the enhancement of enzyme catalyst activity
title_full Characterization and functionalisation of activated carbon for the enhancement of enzyme catalyst activity
title_fullStr Characterization and functionalisation of activated carbon for the enhancement of enzyme catalyst activity
title_full_unstemmed Characterization and functionalisation of activated carbon for the enhancement of enzyme catalyst activity
title_sort characterization and functionalisation of activated carbon for the enhancement of enzyme catalyst activity
publisher Elsevier
publishDate 2021
url http://irep.iium.edu.my/91786/7/91786_Characterization%20and%20functionalisation%20of%20activated%20carbon.pdf
http://irep.iium.edu.my/91786/
https://www.journals.elsevier.com/materials-science-and-engineering-b
_version_ 1709667174770016256