CD4(+)CD25(hi)CD127(low) Regulatory T Cells Are Increased in Oral Squamous Cell Carcinoma Patients

Regulatory T cells (Tregs), a subset of CD4+ T cells plays a pivotal role in regulating the immune system. An increase in Treg numbers enables cancer progression by dampening the immune system and allowing tumor cells to evade immune detection and destruction. An increase in Treg numbers and express...

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Main Authors: Lim, K.P., Chun, N.A.L., Ismail, S.M., Abraham, M.T., Yusoff, M.N., Zain, R.B., Ngeow, Wei Cheong
Format: Article
Language:English
Published: Public Library of Science 2014
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Online Access:http://eprints.um.edu.my/11363/1/fetchObjectAttachment-libre.pdf
http://eprints.um.edu.my/11363/
http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0103975
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spelling my.um.eprints.113632019-09-27T08:53:04Z http://eprints.um.edu.my/11363/ CD4(+)CD25(hi)CD127(low) Regulatory T Cells Are Increased in Oral Squamous Cell Carcinoma Patients Lim, K.P. Chun, N.A.L. Ismail, S.M. Abraham, M.T. Yusoff, M.N. Zain, R.B. Ngeow, Wei Cheong RK Dentistry Regulatory T cells (Tregs), a subset of CD4+ T cells plays a pivotal role in regulating the immune system. An increase in Treg numbers enables cancer progression by dampening the immune system and allowing tumor cells to evade immune detection and destruction. An increase in Treg numbers and expression of inhibitory cytokines including TGF-β and IL-10 are mechanisms by which Tregs exert their immune suppressive function. However, the presence of Tregs and inhibitory cytokines in oral cancer patients is still unclear. In this study, the presence of circulating Tregs in 39 oral cancer patients and 24 healthy donors was examined by studying the presence of the CD4+CD25hiCD127low cell population in their peripheral blood mononuclear cells using flow cytometry. Serum levels of TGF-β and IL-10 were measured by ELISA. T cell subsets of OSCC patients were found to differ significantly from healthy donors where a decrease in CD8+ cytotoxic T cells and an increase in Tregs (CD4+CD25hiCD127low) were observed. Further, the ratio of CD8+ T cells/Tregs was also decreased in patients compared to healthy donors. The presence of Tregs was accompanied by a decrease in IL-10 but not TGF-β secretion in OSCC patients when compared to donors; in addition, the analysis also revealed that an increased presence of Tregs was accompanied by better patient survival. Amongst OSCC patients, smokers had significantly higher levels of TGF-β. It is apparent that the immune system is compromised in OSCC patients and the characterization of the Treg subpopulation could form a basis for improving our understanding of the perturbations in the immune system that occur during OSCC tumorigenesis. Public Library of Science 2014-08-25 Article PeerReviewed application/pdf en http://eprints.um.edu.my/11363/1/fetchObjectAttachment-libre.pdf Lim, K.P. and Chun, N.A.L. and Ismail, S.M. and Abraham, M.T. and Yusoff, M.N. and Zain, R.B. and Ngeow, Wei Cheong (2014) CD4(+)CD25(hi)CD127(low) Regulatory T Cells Are Increased in Oral Squamous Cell Carcinoma Patients. PLoS ONE. ISSN 1932-6203 http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0103975
institution Universiti Malaya
building UM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaya
content_source UM Research Repository
url_provider http://eprints.um.edu.my/
language English
topic RK Dentistry
spellingShingle RK Dentistry
Lim, K.P.
Chun, N.A.L.
Ismail, S.M.
Abraham, M.T.
Yusoff, M.N.
Zain, R.B.
Ngeow, Wei Cheong
CD4(+)CD25(hi)CD127(low) Regulatory T Cells Are Increased in Oral Squamous Cell Carcinoma Patients
description Regulatory T cells (Tregs), a subset of CD4+ T cells plays a pivotal role in regulating the immune system. An increase in Treg numbers enables cancer progression by dampening the immune system and allowing tumor cells to evade immune detection and destruction. An increase in Treg numbers and expression of inhibitory cytokines including TGF-β and IL-10 are mechanisms by which Tregs exert their immune suppressive function. However, the presence of Tregs and inhibitory cytokines in oral cancer patients is still unclear. In this study, the presence of circulating Tregs in 39 oral cancer patients and 24 healthy donors was examined by studying the presence of the CD4+CD25hiCD127low cell population in their peripheral blood mononuclear cells using flow cytometry. Serum levels of TGF-β and IL-10 were measured by ELISA. T cell subsets of OSCC patients were found to differ significantly from healthy donors where a decrease in CD8+ cytotoxic T cells and an increase in Tregs (CD4+CD25hiCD127low) were observed. Further, the ratio of CD8+ T cells/Tregs was also decreased in patients compared to healthy donors. The presence of Tregs was accompanied by a decrease in IL-10 but not TGF-β secretion in OSCC patients when compared to donors; in addition, the analysis also revealed that an increased presence of Tregs was accompanied by better patient survival. Amongst OSCC patients, smokers had significantly higher levels of TGF-β. It is apparent that the immune system is compromised in OSCC patients and the characterization of the Treg subpopulation could form a basis for improving our understanding of the perturbations in the immune system that occur during OSCC tumorigenesis.
format Article
author Lim, K.P.
Chun, N.A.L.
Ismail, S.M.
Abraham, M.T.
Yusoff, M.N.
Zain, R.B.
Ngeow, Wei Cheong
author_facet Lim, K.P.
Chun, N.A.L.
Ismail, S.M.
Abraham, M.T.
Yusoff, M.N.
Zain, R.B.
Ngeow, Wei Cheong
author_sort Lim, K.P.
title CD4(+)CD25(hi)CD127(low) Regulatory T Cells Are Increased in Oral Squamous Cell Carcinoma Patients
title_short CD4(+)CD25(hi)CD127(low) Regulatory T Cells Are Increased in Oral Squamous Cell Carcinoma Patients
title_full CD4(+)CD25(hi)CD127(low) Regulatory T Cells Are Increased in Oral Squamous Cell Carcinoma Patients
title_fullStr CD4(+)CD25(hi)CD127(low) Regulatory T Cells Are Increased in Oral Squamous Cell Carcinoma Patients
title_full_unstemmed CD4(+)CD25(hi)CD127(low) Regulatory T Cells Are Increased in Oral Squamous Cell Carcinoma Patients
title_sort cd4(+)cd25(hi)cd127(low) regulatory t cells are increased in oral squamous cell carcinoma patients
publisher Public Library of Science
publishDate 2014
url http://eprints.um.edu.my/11363/1/fetchObjectAttachment-libre.pdf
http://eprints.um.edu.my/11363/
http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0103975
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