Optimised conditions for Pulsed Field Gel Electrophoresis (PFGE) separations of Pasteurella multocida B:2 DNA
Pulsed-field condition specific for Pasteurella multocida B:2 have been optimized to obtain a good separation resolution. Restriction enzyme, NotI, was the most suitable as it generates discernible number of bands. Other optimised electrophoretic conditions were: 1.5% agarose gel concentration, 200V...
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Main Authors: | , , , |
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Format: | Article |
Language: | English |
Published: |
Malaysian Society for Molecular Biology and Biotechnology
2001
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Subjects: | |
Online Access: | http://eprints.um.edu.my/9969/1/Hasima_et_al.%2C_2001.pdf http://eprints.um.edu.my/9969/ http://www.msmbb.my/index.php/archive-issues |
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Institution: | Universiti Malaya |
Language: | English |