Optimised conditions for Pulsed Field Gel Electrophoresis (PFGE) separations of Pasteurella multocida B:2 DNA

• Main Authors: Hasima, N.N., Saidi, J., Thong, Kwai Lin, Ansary, A.
• Format: Article
• Language: English
• Published: Malaysian Society for Molecular Biology and Biotechnology 2001
• Subjects: QR Microbiology
• Online Access: http://eprints.um.edu.my/9969/1/Hasima_et_al.%2C_2001.pdf; http://eprints.um.edu.my/9969/; http://www.msmbb.my/index.php/archive-issues
• Institution: Universiti Malaya

Pulsed-field condition specific for Pasteurella multocida B:2 have been optimized to obtain a good separation resolution. Restriction enzyme, NotI, was the most suitable as it generates discernible number of bands. Other optimised electrophoretic conditions were: 1.5% agarose gel concentration, 200V, ramped pulse time of 1-25 s, and a runtime of 26 h. This research is important for fast and accurate characterisation of this bacterial strain following an outbreak in Malaysia.