Protein profiling of flesh in mud of crab (scylla serrata)
Protein profiling is a direct measurement of proteins in terms of their presence and relative abundance. Flesh of mud crab. Scylla serrata, consists of various protein as well as enzymes. The approaches used to carry out protein profiling of Mud Crab, Scylla serrata, in flesh was Sodium Dodecyl Sulf...
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| Format: | Undergraduate Final Project Report |
| Published: |
2013
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| Online Access: | http://discol.umk.edu.my/id/eprint/5721/ |
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| Institution: | Universiti Malaysia Kelantan |
| Summary: | Protein profiling is a direct measurement of proteins in terms of their presence and relative abundance. Flesh of mud crab. Scylla serrata, consists of various protein as well as enzymes. The approaches used to carry out protein profiling of Mud Crab, Scylla serrata, in flesh was Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE). The application of this method involved the induction of negatively-charged ions to the samples in order to separate protein solely based on sizes. The determination of molecular weight of unknown proteins was done via the application of Image J Software. Protein Profiling of Mud Crab, Scylla serrata, in flesh had shown 11 bands each from 8%, 10% and 12% gel percentage from 21.5 to 119.3 kDa, 14.4 to 100.1 kDa and 14.4 to 83.8 kDa, respectively. |
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