An improved technique for the rapid chemical characterisation of bacterial terpene cyclases

An improved technique for the rapid chemical characterisation of bacterial terpene cyclases

A derivative of the pET28c(+) expression vector was constructed. It contains a yeast replication system (2μ origin of replication) and a yeast selectable marker (URA3), and can be used for gene cloning in yeast by efficient homologous recombination, and for heterologous expression in E. coli. The ve...

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Main Authors: Jeroen S. Dickschat, Khomaizon A. K. Pahirulzaman, Patrick Rabe, Tim A. Klapschinski
Format: Non-Indexed Article
Published: 2014
Online Access:http://discol.umk.edu.my/id/eprint/8102/
http://onlinelibrary.wiley.com/doi/10.1002/cbic.201300763/abstract;jsessionid=410C17355B17CC08D35F227893B84109.f01t01?userIsAuthenticated=false&deniedAccessCustomisedMessage=
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Institution: Universiti Malaysia Kelantan
id my.umk.eprints.8102
record_format eprints
spelling my.umk.eprints.81022022-05-23T10:25:17Z http://discol.umk.edu.my/id/eprint/8102/ An improved technique for the rapid chemical characterisation of bacterial terpene cyclases Jeroen S. Dickschat Khomaizon A. K. Pahirulzaman Patrick Rabe Tim A. Klapschinski A derivative of the pET28c(+) expression vector was constructed. It contains a yeast replication system (2μ origin of replication) and a yeast selectable marker (URA3), and can be used for gene cloning in yeast by efficient homologous recombination, and for heterologous expression in E. coli. The vector was used for the expression and chemical characterisation of three bacterial terpene cyclases. 2014 Non-Indexed Article NonPeerReviewed Jeroen S. Dickschat and Khomaizon A. K. Pahirulzaman and Patrick Rabe and Tim A. Klapschinski (2014) An improved technique for the rapid chemical characterisation of bacterial terpene cyclases. ChemBioChem, 15 (6). pp. 810-814. ISSN 1439-7633 http://onlinelibrary.wiley.com/doi/10.1002/cbic.201300763/abstract;jsessionid=410C17355B17CC08D35F227893B84109.f01t01?userIsAuthenticated=false&deniedAccessCustomisedMessage=
institution Universiti Malaysia Kelantan
building Perpustakaan Universiti Malaysia Kelantan
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Kelantan
content_source UMK Institutional Repository
url_provider http://umkeprints.umk.edu.my/
description A derivative of the pET28c(+) expression vector was constructed. It contains a yeast replication system (2μ origin of replication) and a yeast selectable marker (URA3), and can be used for gene cloning in yeast by efficient homologous recombination, and for heterologous expression in E. coli. The vector was used for the expression and chemical characterisation of three bacterial terpene cyclases.
format Non-Indexed Article
author Jeroen S. Dickschat
Khomaizon A. K. Pahirulzaman
Patrick Rabe
Tim A. Klapschinski
spellingShingle Jeroen S. Dickschat
Khomaizon A. K. Pahirulzaman
Patrick Rabe
Tim A. Klapschinski
An improved technique for the rapid chemical characterisation of bacterial terpene cyclases
author_facet Jeroen S. Dickschat
Khomaizon A. K. Pahirulzaman
Patrick Rabe
Tim A. Klapschinski
author_sort Jeroen S. Dickschat
title An improved technique for the rapid chemical characterisation of bacterial terpene cyclases
title_short An improved technique for the rapid chemical characterisation of bacterial terpene cyclases
title_full An improved technique for the rapid chemical characterisation of bacterial terpene cyclases
title_fullStr An improved technique for the rapid chemical characterisation of bacterial terpene cyclases
title_full_unstemmed An improved technique for the rapid chemical characterisation of bacterial terpene cyclases
title_sort improved technique for the rapid chemical characterisation of bacterial terpene cyclases
publishDate 2014
url http://discol.umk.edu.my/id/eprint/8102/
http://onlinelibrary.wiley.com/doi/10.1002/cbic.201300763/abstract;jsessionid=410C17355B17CC08D35F227893B84109.f01t01?userIsAuthenticated=false&deniedAccessCustomisedMessage=
_version_ 1763303936036438016

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