An improved technique for the rapid chemical characterisation of bacterial terpene cyclases

• Main Authors: Jeroen S. Dickschat, Khomaizon A. K. Pahirulzaman, Patrick Rabe, Tim A. Klapschinski
• Format: Non-Indexed Article
• Published: 2014
• Online Access: http://discol.umk.edu.my/id/eprint/8102/; http://onlinelibrary.wiley.com/doi/10.1002/cbic.201300763/abstract;jsessionid=410C17355B17CC08D35F227893B84109.f01t01?userIsAuthenticated=false&deniedAccessCustomisedMessage=
• Institution: Universiti Malaysia Kelantan

A derivative of the pET28c(+) expression vector was constructed. It contains a yeast replication system (2μ origin of replication) and a yeast selectable marker (URA3), and can be used for gene cloning in yeast by efficient homologous recombination, and for heterologous expression in E. coli. The ve...