The effect of selected plant extracts on the expression prifiles of the insulin 2 gene in pancreatic cells of streptozotocin-induced diabetic rats
Diabetes is a major public health problem. The new therapies developments that are able to Improve glycemia management, cure diabetes and even protect from it are of great interest. This study investigated the effect of selected plant extracts on the Insulin gene expression profile against STZ- i...
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| Main Author: | |
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| Format: | Thesis |
| Language: | English |
| Published: |
2014
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| Subjects: | |
| Online Access: | https://eprints.ums.edu.my/id/eprint/19571/1/The%20effect%20of%20selected%20plant%20extracts.pdf https://eprints.ums.edu.my/id/eprint/19571/ |
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| Institution: | Universiti Malaysia Sabah |
| Language: | English |
| Summary: | Diabetes is a major public health problem. The new therapies developments that are
able to Improve glycemia management, cure diabetes and even protect from it are of
great interest. This study investigated the effect of selected plant extracts on the
Insulin gene expression profile against STZ- induced pancreatic cell damages.
Eighteen rats were divided into six groups: (i) Nonnal control rats; (ii) diabetic
control rats, diabetic rats administered with (iii) Momorriica Charantia, (iv) Morinda
Citrifolia, (v) Parkia Speciosa and (vi)Metformin. All rats were given treatment daily
for 3 days before the STZ injection and for 1 subsequent week thereafter. M.
charantia extract showed the highest significant value of total phenolic content
(292.13 mg/g) compared to M. citrifolia and P. speciosa extracts. The DPPH
scavenging rate for M. charantia extract was ranging from 4.3% to 51.8% followed
by M. dtrifolia extracts (1.8% to 57.9%) and P. speciosa extract (0.4% to 48.7%).
The result showed that M. charantia extract has the highest antioxidant activity with
EC₅₀ value was 356.56 µg/ mi. M. charantia extract also showed the highest value in
the total flavanoid content (14.56 mg/g) compared to other two samples. Expression
of both GAPDH as the reference gene and Ins2 gene as the target gene was
measured by SYBR Green I fluorescence; melting curves and agarose gel
electrophoresis were used to verify the specificity of gene (Ins2) for diabetic rats
treated with M. charantia extracts expressing fold differences of 32 fold expression
compare to the diabetic control rats only. This study report the preliminary study of
the effect of plant extracts on the pancreatic cell insulin gene expression profile. The
results represent an important step toward improved understanding of the regulation
of insulin secretion on insulin 2 (1ns2) genes. |
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