The effect of selected plant extracts on the expression prifiles of the insulin 2 gene in pancreatic cells of streptozotocin-induced diabetic rats

• Main Author: Valentino Milton Junior Gumbilai
• Format: Thesis
• Language: English
• Published: 2014
• Subjects: RC Internal medicine
• Online Access: https://eprints.ums.edu.my/id/eprint/19571/1/The%20effect%20of%20selected%20plant%20extracts.pdf; https://eprints.ums.edu.my/id/eprint/19571/
• Institution: Universiti Malaysia Sabah

Diabetes is a major public health problem. The new therapies developments that are able to Improve glycemia management, cure diabetes and even protect from it are of great interest. This study investigated the effect of selected plant extracts on the Insulin gene expression profile against STZ- induced pancreatic cell damages. Eighteen rats were divided into six groups: (i) Nonnal control rats; (ii) diabetic control rats, diabetic rats administered with (iii) Momorriica Charantia, (iv) Morinda Citrifolia, (v) Parkia Speciosa and (vi)Metformin. All rats were given treatment daily for 3 days before the STZ injection and for 1 subsequent week thereafter. M. charantia extract showed the highest significant value of total phenolic content (292.13 mg/g) compared to M. citrifolia and P. speciosa extracts. The DPPH scavenging rate for M. charantia extract was ranging from 4.3% to 51.8% followed by M. dtrifolia extracts (1.8% to 57.9%) and P. speciosa extract (0.4% to 48.7%). The result showed that M. charantia extract has the highest antioxidant activity with EC₅₀ value was 356.56 µg/ mi. M. charantia extract also showed the highest value in the total flavanoid content (14.56 mg/g) compared to other two samples. Expression of both GAPDH as the reference gene and Ins2 gene as the target gene was measured by SYBR Green I fluorescence; melting curves and agarose gel electrophoresis were used to verify the specificity of gene (Ins2) for diabetic rats treated with M. charantia extracts expressing fold differences of 32 fold expression compare to the diabetic control rats only. This study report the preliminary study of the effect of plant extracts on the pancreatic cell insulin gene expression profile. The results represent an important step toward improved understanding of the regulation of insulin secretion on insulin 2 (1ns2) genes.