Nanobiosensor for the detection and quantification of specific DNA sequences in degraded biological samples
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Springer-Verlag
2011
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my.unimap-140112011-10-04T12:57:46Z Nanobiosensor for the detection and quantification of specific DNA sequences in degraded biological samples M. E., Ali Uda, Hashim, Prof. Dr. Shuhaimi, Mustafa Yaakob, Che Man, Dato' Dr. M. H. M., Yusop Hybrid nanobioprobe Hybridization kinetics Sigmoidal relationship Species specific nanobiosensor Synthetic oligo-targets Link to publisher's homepage at http://www.springerlink.com/ A 27-nucleotide AluI fragment of swine cytochrome b (cytb) gene was integrated to a 3-nm diameter citrate- tannate coated gold nanoparticle to fabricate a species specific nanobiosensor. The biosensor was applied to authenticate pork adulteration in autoclaved pork-beef mixtures. The sensor was found to be sensitive enough to detect 0.5 pork in raw and 2.5-h autoclaved mixed samples in a single step without any separation or washing. The hybridization kinetics of the hybrid sensor was studied with synthetic targets from moderate to extreme target concentrations and a sigmoidal relationship was found. The kinetic curve was used to develop a convenient method for quantifying and counting target DNA copy number. The biosensor probe was hybridized with a target DNA that was several-folds shorter than a typical PCR-template. This offered the detection and quantitation of potential targets in highly processed meat products or extensively degraded samples where PCR-based identification technique might not work due to the degradation of comparatively longer DNA. The assay was a viable alternative approach of qPCR for detecting, quantifying and counting copy number of shorter size DNA sequences in degraded samples to address a range of biological problems such as food analysis, biodiagnostics, environmental monitoring, genetic screening and forensic investigations. 2011-10-04T12:57:46Z 2011-10-04T12:57:46Z 2011-06-20 Working Paper p. 384-387 978-3-6422-1728-9 1680-0737 http://www.springerlink.com/content/v1g542165vx74353/fulltext.pdf http://hdl.handle.net/123456789/14011 en Proceedings of the 5th Kuala Lumpur International Conference on Biomedical Engineering (BioMed 2011) Springer-Verlag |
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Universiti Malaysia Perlis |
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Universiti Malaysia Perlis |
| content_source |
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| language |
English |
| topic |
Hybrid nanobioprobe Hybridization kinetics Sigmoidal relationship Species specific nanobiosensor Synthetic oligo-targets |
| spellingShingle |
Hybrid nanobioprobe Hybridization kinetics Sigmoidal relationship Species specific nanobiosensor Synthetic oligo-targets M. E., Ali Uda, Hashim, Prof. Dr. Shuhaimi, Mustafa Yaakob, Che Man, Dato' Dr. M. H. M., Yusop Nanobiosensor for the detection and quantification of specific DNA sequences in degraded biological samples |
| description |
Link to publisher's homepage at http://www.springerlink.com/ |
| format |
Working Paper |
| author |
M. E., Ali Uda, Hashim, Prof. Dr. Shuhaimi, Mustafa Yaakob, Che Man, Dato' Dr. M. H. M., Yusop |
| author_facet |
M. E., Ali Uda, Hashim, Prof. Dr. Shuhaimi, Mustafa Yaakob, Che Man, Dato' Dr. M. H. M., Yusop |
| author_sort |
M. E., Ali |
| title |
Nanobiosensor for the detection and quantification of specific DNA sequences in degraded biological samples |
| title_short |
Nanobiosensor for the detection and quantification of specific DNA sequences in degraded biological samples |
| title_full |
Nanobiosensor for the detection and quantification of specific DNA sequences in degraded biological samples |
| title_fullStr |
Nanobiosensor for the detection and quantification of specific DNA sequences in degraded biological samples |
| title_full_unstemmed |
Nanobiosensor for the detection and quantification of specific DNA sequences in degraded biological samples |
| title_sort |
nanobiosensor for the detection and quantification of specific dna sequences in degraded biological samples |
| publisher |
Springer-Verlag |
| publishDate |
2011 |
| url |
http://dspace.unimap.edu.my/xmlui/handle/123456789/14011 |
| _version_ |
1643790965343780864 |