Gold nanoparticle sensor for the visual detection of pork adulteration in meatball formulation
Link to publisher's homepage at http://www.hindawi.com/
Saved in:
| Main Authors: | , , , , |
|---|---|
| Other Authors: | |
| Format: | Article |
| Language: | English |
| Published: |
Hindawi Publishing Corporation
2013
|
| Subjects: | |
| Online Access: | http://dspace.unimap.edu.my/xmlui/handle/123456789/26005 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Institution: | Universiti Malaysia Perlis |
| Language: | English |
| id |
my.unimap-26005 |
|---|---|
| record_format |
dspace |
| spelling |
my.unimap-260052013-06-21T09:38:05Z Gold nanoparticle sensor for the visual detection of pork adulteration in meatball formulation Md Eaqub, Ali Uda, Hashim, Prof. Dr. Shuhaimi, Mustafa, Assoc. Prof. Dr. Yaakob, Che Man, Prof. Datuk Dr. Kh. N. Islam uda@unimap.edu.my Gold nanoparticles (GNPs) Pork adulteration Food analysis Link to publisher's homepage at http://www.hindawi.com/ We visually identify pork adulteration in beef and chicken meatball preparations using 20 nm gold nanoparticles (GNPs) as colorimetric sensors. Meatball is a popular food in certain Asian and European countries. Verification of pork adulteration in meatball is necessary to meet the Halal and Kosher food standards. Twenty nm GNPs change color from pinkish-red to gray-purple, and their absorption peak at 525 nm is red-shifted by 30–50 nm in 3 mM phosphate buffer saline (PBS). Adsorption of single-stranded DNA protects the particles against salt-induced aggregation. Mixing and annealing of a 25-nucleotide (nt) single-stranded (ss) DNA probe with denatured DNA of different meatballs differentiated well between perfectly matched and mismatch hybridization at a critical annealing temperature. The probes become available in nonpork DNA containing vials due to mismatches and interact with GNPs to protect them from salt-induced aggregation. Whereas, all the pork containing vials, either in pure and mixed forms, consumed the probes totally by perfect hybridization and turned into grey, indicating aggregation. This is clearly reflected by a well-defined red-shift of the absorption peak and significantly increased absorbance in 550–800 nm regimes. This label-free low-cost assay should find applications in food analysis, genetic screening, and homology studies. 2013-06-21T09:38:05Z 2013-06-21T09:38:05Z 2012 Article Journal of Nanomaterials, vol. 2012, 2012, pages 1-7 1687-4110 http://www.hindawi.com/journals/jnm/2012/103607/ http://hdl.handle.net/123456789/26005 en Hindawi Publishing Corporation |
| institution |
Universiti Malaysia Perlis |
| building |
UniMAP Library |
| collection |
Institutional Repository |
| continent |
Asia |
| country |
Malaysia |
| content_provider |
Universiti Malaysia Perlis |
| content_source |
UniMAP Library Digital Repository |
| url_provider |
http://dspace.unimap.edu.my/ |
| language |
English |
| topic |
Gold nanoparticles (GNPs) Pork adulteration Food analysis |
| spellingShingle |
Gold nanoparticles (GNPs) Pork adulteration Food analysis Md Eaqub, Ali Uda, Hashim, Prof. Dr. Shuhaimi, Mustafa, Assoc. Prof. Dr. Yaakob, Che Man, Prof. Datuk Dr. Kh. N. Islam Gold nanoparticle sensor for the visual detection of pork adulteration in meatball formulation |
| description |
Link to publisher's homepage at http://www.hindawi.com/ |
| author2 |
uda@unimap.edu.my |
| author_facet |
uda@unimap.edu.my Md Eaqub, Ali Uda, Hashim, Prof. Dr. Shuhaimi, Mustafa, Assoc. Prof. Dr. Yaakob, Che Man, Prof. Datuk Dr. Kh. N. Islam |
| format |
Article |
| author |
Md Eaqub, Ali Uda, Hashim, Prof. Dr. Shuhaimi, Mustafa, Assoc. Prof. Dr. Yaakob, Che Man, Prof. Datuk Dr. Kh. N. Islam |
| author_sort |
Md Eaqub, Ali |
| title |
Gold nanoparticle sensor for the visual detection of pork adulteration in meatball formulation |
| title_short |
Gold nanoparticle sensor for the visual detection of pork adulteration in meatball formulation |
| title_full |
Gold nanoparticle sensor for the visual detection of pork adulteration in meatball formulation |
| title_fullStr |
Gold nanoparticle sensor for the visual detection of pork adulteration in meatball formulation |
| title_full_unstemmed |
Gold nanoparticle sensor for the visual detection of pork adulteration in meatball formulation |
| title_sort |
gold nanoparticle sensor for the visual detection of pork adulteration in meatball formulation |
| publisher |
Hindawi Publishing Corporation |
| publishDate |
2013 |
| url |
http://dspace.unimap.edu.my/xmlui/handle/123456789/26005 |
| _version_ |
1643794807375527936 |