Optimisation of biodegradation conditions for cyanide removal by Serratia marcescens strain AQ07 using one-factor-at-a-time technique and response surface methodology behavior
Gold mining companies are known to use cyanide to extract gold from minerals. The indiscriminate use of cyanide presents a major environmental issue. Serratia marcescens strain AQ07 was found to have cyanidedegrading ability. Optimisation of biodegradation condition was carried out utilising on...
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Main Authors: | , , , , , , , |
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Format: | E-Article |
Language: | English |
Published: |
Springer International Publishing
2016
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Subjects: | |
Online Access: | http://ir.unimas.my/id/eprint/11464/1/Optimisation%20of%20biodegradation%20conditions%20for%20cyanide%20removal%20%28abstract%29.pdf http://ir.unimas.my/id/eprint/11464/ http://link.springer.com/article/10.1007%2Fs12210-016-0516-8 |
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Institution: | Universiti Malaysia Sarawak |
Language: | English |
Summary: | Gold mining companies are known to use
cyanide to extract gold from minerals. The indiscriminate
use of cyanide presents a major environmental issue. Serratia
marcescens strain AQ07 was found to have cyanidedegrading
ability. Optimisation of biodegradation condition
was carried out utilising one factor at a time and
response surface methodology. Cyanide degradation corresponded
with growth rate with a maximum growth rate of
16.14 log cfu/mL on day 3 of incubation. Glucose and
yeast extract are suitable carbon and nitrogen sources. Six
parameters including carbon and nitrogen sources, pH,
temperature, inoculum size and cyanide concentration were
optimised. In line with the central composite design of
response surface methodology, cyanide degradation was
optimum at glucose concentration 5.5 g/L, yeast extract
0.55 g/L, pH 6, temperature 32.5 �C, inoculum size 20 %
and cyanide concentration 200 mg/L. It was able to stand
cyanide toxicity of up to 700 mg/L, which makes it an
important candidate for bioremediation of cyanide. The
bacterium was observed to degrade 95.6 % of 200 mg/L
KCN under the optimised condition. Bacteria are reported
to degrade cyanide into ammonia, formamide or formate
and carbon dioxide, which are less toxic by-products.
These bacteria illustrate good cyanide degradation potential
that can be harnessed in cyanide remediation. |
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