Optimisation of biodegradation conditions for cyanide removal by Serratia marcescens strain AQ07 using one-factor-at-a-time technique and response surface methodology behavior
Gold mining companies are known to use cyanide to extract gold from minerals. The indiscriminate use of cyanide presents a major environmental issue. Serratia marcescens strain AQ07 was found to have cyanidedegrading ability. Optimisation of biodegradation condition was carried out utilising on...
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my.unimas.ir.114642016-11-17T04:15:32Z http://ir.unimas.my/id/eprint/11464/ Optimisation of biodegradation conditions for cyanide removal by Serratia marcescens strain AQ07 using one-factor-at-a-time technique and response surface methodology behavior Kabiru Ibrahim, Karamba Siti Aqlima, binti Ahmad Azham, Zulkharnain Mohd Arif, Syed Khalilah, Abdul Khalil Nor Aripin, Shamaan Farrah Aini, Dahalan Mohd Yunus, Shukor GE Environmental Sciences Gold mining companies are known to use cyanide to extract gold from minerals. The indiscriminate use of cyanide presents a major environmental issue. Serratia marcescens strain AQ07 was found to have cyanidedegrading ability. Optimisation of biodegradation condition was carried out utilising one factor at a time and response surface methodology. Cyanide degradation corresponded with growth rate with a maximum growth rate of 16.14 log cfu/mL on day 3 of incubation. Glucose and yeast extract are suitable carbon and nitrogen sources. Six parameters including carbon and nitrogen sources, pH, temperature, inoculum size and cyanide concentration were optimised. In line with the central composite design of response surface methodology, cyanide degradation was optimum at glucose concentration 5.5 g/L, yeast extract 0.55 g/L, pH 6, temperature 32.5 �C, inoculum size 20 % and cyanide concentration 200 mg/L. It was able to stand cyanide toxicity of up to 700 mg/L, which makes it an important candidate for bioremediation of cyanide. The bacterium was observed to degrade 95.6 % of 200 mg/L KCN under the optimised condition. Bacteria are reported to degrade cyanide into ammonia, formamide or formate and carbon dioxide, which are less toxic by-products. These bacteria illustrate good cyanide degradation potential that can be harnessed in cyanide remediation. Springer International Publishing 2016 E-Article PeerReviewed text en http://ir.unimas.my/id/eprint/11464/1/Optimisation%20of%20biodegradation%20conditions%20for%20cyanide%20removal%20%28abstract%29.pdf Kabiru Ibrahim, Karamba and Siti Aqlima, binti Ahmad and Azham, Zulkharnain and Mohd Arif, Syed and Khalilah, Abdul Khalil and Nor Aripin, Shamaan and Farrah Aini, Dahalan and Mohd Yunus, Shukor (2016) Optimisation of biodegradation conditions for cyanide removal by Serratia marcescens strain AQ07 using one-factor-at-a-time technique and response surface methodology behavior. Rendiconti Lincei, 27 (3). pp. 533-545. ISSN 1720-0776 http://link.springer.com/article/10.1007%2Fs12210-016-0516-8 DOI 10.1007/s12210-016-0516-8 |
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GE Environmental Sciences Kabiru Ibrahim, Karamba Siti Aqlima, binti Ahmad Azham, Zulkharnain Mohd Arif, Syed Khalilah, Abdul Khalil Nor Aripin, Shamaan Farrah Aini, Dahalan Mohd Yunus, Shukor Optimisation of biodegradation conditions for cyanide removal by Serratia marcescens strain AQ07 using one-factor-at-a-time technique and response surface methodology behavior |
description |
Gold mining companies are known to use
cyanide to extract gold from minerals. The indiscriminate
use of cyanide presents a major environmental issue. Serratia
marcescens strain AQ07 was found to have cyanidedegrading
ability. Optimisation of biodegradation condition
was carried out utilising one factor at a time and
response surface methodology. Cyanide degradation corresponded
with growth rate with a maximum growth rate of
16.14 log cfu/mL on day 3 of incubation. Glucose and
yeast extract are suitable carbon and nitrogen sources. Six
parameters including carbon and nitrogen sources, pH,
temperature, inoculum size and cyanide concentration were
optimised. In line with the central composite design of
response surface methodology, cyanide degradation was
optimum at glucose concentration 5.5 g/L, yeast extract
0.55 g/L, pH 6, temperature 32.5 �C, inoculum size 20 %
and cyanide concentration 200 mg/L. It was able to stand
cyanide toxicity of up to 700 mg/L, which makes it an
important candidate for bioremediation of cyanide. The
bacterium was observed to degrade 95.6 % of 200 mg/L
KCN under the optimised condition. Bacteria are reported
to degrade cyanide into ammonia, formamide or formate
and carbon dioxide, which are less toxic by-products.
These bacteria illustrate good cyanide degradation potential
that can be harnessed in cyanide remediation. |
format |
E-Article |
author |
Kabiru Ibrahim, Karamba Siti Aqlima, binti Ahmad Azham, Zulkharnain Mohd Arif, Syed Khalilah, Abdul Khalil Nor Aripin, Shamaan Farrah Aini, Dahalan Mohd Yunus, Shukor |
author_facet |
Kabiru Ibrahim, Karamba Siti Aqlima, binti Ahmad Azham, Zulkharnain Mohd Arif, Syed Khalilah, Abdul Khalil Nor Aripin, Shamaan Farrah Aini, Dahalan Mohd Yunus, Shukor |
author_sort |
Kabiru Ibrahim, Karamba |
title |
Optimisation of biodegradation conditions for cyanide removal by Serratia marcescens strain AQ07 using one-factor-at-a-time technique and response surface methodology behavior |
title_short |
Optimisation of biodegradation conditions for cyanide removal by Serratia marcescens strain AQ07 using one-factor-at-a-time technique and response surface methodology behavior |
title_full |
Optimisation of biodegradation conditions for cyanide removal by Serratia marcescens strain AQ07 using one-factor-at-a-time technique and response surface methodology behavior |
title_fullStr |
Optimisation of biodegradation conditions for cyanide removal by Serratia marcescens strain AQ07 using one-factor-at-a-time technique and response surface methodology behavior |
title_full_unstemmed |
Optimisation of biodegradation conditions for cyanide removal by Serratia marcescens strain AQ07 using one-factor-at-a-time technique and response surface methodology behavior |
title_sort |
optimisation of biodegradation conditions for cyanide removal by serratia marcescens strain aq07 using one-factor-at-a-time technique and response surface methodology behavior |
publisher |
Springer International Publishing |
publishDate |
2016 |
url |
http://ir.unimas.my/id/eprint/11464/1/Optimisation%20of%20biodegradation%20conditions%20for%20cyanide%20removal%20%28abstract%29.pdf http://ir.unimas.my/id/eprint/11464/ http://link.springer.com/article/10.1007%2Fs12210-016-0516-8 |
_version_ |
1644511221521580032 |