Recombination between linear double-stranded DNA substrates in vivo
Recombineering technology in E. coli enables targeting of linear donor DNA to circular recipient DNA using short shared homology sequences. In this work, we demonstrate that recombineering is also able to support recombination between a pair of linear DNA substrates (linear/linear recombineering)...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Academic Press Inc.
2009
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| Subjects: | |
| Online Access: | http://ir.unimas.my/id/eprint/16918/1/Kumaran.pdf http://ir.unimas.my/id/eprint/16918/ http://www.sciencedirect.com/science/journal/00032697?sdc=1 |
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| Institution: | Universiti Malaysia Sarawak |
| Language: | English |
| Summary: | Recombineering technology in E. coli enables targeting of linear donor DNA to circular recipient
DNA using short shared homology sequences. In this work, we demonstrate that recombineering is
also able to support recombination between a pair of linear DNA substrates (linear/linear
recombineering) in vivo in E. coli. Linear DNA up to 100 kb is accurately modified and remains
intact without undergoing rearrangements after recombination. This system will be valuable for direct
in vivo manipulation of large linear DNA including the N15 and PY54 prophages and linear animal
viruses, and for assembly of linear constructs as artificial chromosome vectors. |
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