Recombination between linear double-stranded DNA substrates in vivo

• Main Authors: Narayanan, Kulathuramaiyer, Sim, Edmund U. H., Ravin, Nikolai V., Choon, Weng Lee
• Format: Article
• Language: English
• Published: Academic Press Inc. 2009
• Subjects: QD Chemistry; R Medicine (General)
• Online Access: http://ir.unimas.my/id/eprint/16918/1/Kumaran.pdf; http://ir.unimas.my/id/eprint/16918/; http://www.sciencedirect.com/science/journal/00032697?sdc=1
• Institution: Universiti Malaysia Sarawak

Recombineering technology in E. coli enables targeting of linear donor DNA to circular recipient DNA using short shared homology sequences. In this work, we demonstrate that recombineering is also able to support recombination between a pair of linear DNA substrates (linear/linear recombineering)...