HIF-1 sensor in detecting hypoxia tolerance at high altitude

• Main Authors: Shaharuddin, Shazreen, Nik Abd Rahman, Nik Mohd Afizan, Masarudin, Mas Jaffri, Alamassi, Mohammedarfat N., Ahmad Saad, Fathinul Fikri
• Format: Article
• Published: Aerospace Medical Association 2023
• Online Access: http://psasir.upm.edu.my/id/eprint/108173/; https://www.ingentaconnect.com/content/asma/amhp/2023/00000094/00000006/art00014;jsessionid=71ydx8oeue8j.x-ic-live-03
• Institution: Universiti Putra Malaysia

INTRODUCTION: An episode of prolonged exposure to high altitude can cause hypoxia and have significant health consequences. In people with a high-altitude disorder, the body reacts by producing a protein called hypoxia-inducible factor (HIF), which triggers a series of physiological changes and serves a central role in the hypoxia response. Its activity is regulated by the oxygen-dependent degradation of the HIF-1α protein (HIF-1A gene). Therefore, the effects of low oxygen tension in high altitude were explored using fluorescent sensors of hypoxia. METHODS: The development of the sensor provided more sensitivity for detecting hypoxia by generating a calibration of optimized parameters such as reagent concentrations, reagent volumes, and device dimensions. RESULTS: There is a high sensitivity and specificity in detecting the changes of HIF-1α protein hypoxia using the feasibility hypoxia test. This would enable point-of-care (POC) testing and individual self-administration, resulting in faster and more accurate results that can be used for a robust diagnostic approach and enhanced health surveillance, particularly in high-altitude exposure.