Statistical optimization of the induction of the induction of phytase production by Arabinose in a recombinant E. coli using response surface methodology

• Main Authors: Farouk, Abd-ElAziem, Meor Hussin, Anis Shobirin, Greiner, Ralf, Ismail, Shareef Mohideen, Batcha, Mohamed Faizal, Mohd Nur Lubis, Hamadah
• Format: Article
• Language: English; English
• Published: ThaiScience 2009
• Online Access: http://psasir.upm.edu.my/id/eprint/16840/1/Statistical%20optimization%20of%20the%20induction%20of%20the%20induction%20of%20phytase%20production%20by%20Arabinose%20in%20a%20recombinant%20E.pdf; http://psasir.upm.edu.my/id/eprint/16840/; http://www.thaiscience.info/journals/view3.asp?sCode=ASTD&sType=JOURNAL#Year 2009, Volume 26
• Institution: Universiti Putra Malaysia

The production of phytase in a recombinant E.coli using the pBAD expression system was optimized using response surface methodology with full-factorial faced centered central composite design. The ampicilin and arabinose concentration in the cultivation media and the incubation temperatu...