Effect of Bcl-2 overexpression on cell cycle and antibody productivity in chemostat cultures of myeloma NS0 cells

• Main Authors: Tey, Beng Ti, Al-Rubeai, Mohamed
• Format: Article
• Language: English; English
• Published: The Society for Biotechnology 2005
• Online Access: http://psasir.upm.edu.my/id/eprint/18322/1/Effect%20of%20Bcl.pdf; http://psasir.upm.edu.my/id/eprint/18322/; http://dx.doi.org/10.1263/jbb.100.303
• Institution: Universiti Putra Malaysia

Chemostat cultures of NS0 cell lines were carried out at dilution rates ranging from 0.8 d-1 to 0.2 d-1. Compared with the control, the viable cell density of the Bcl-2 cell line was approximately 10% higher at 0.8 d-1 and increased to 55% when the dilution rate was reduced to 0.2 d-1. As the dilution rate was reduced, the viability of the two cultures diverged reaching a difference of 43% at 0.2 d-1. The specific growth rate of the control cells was the same as the dilution rate down to a value of 0.6 d-1. By contrast, the specific growth rate of Bcl-2 cells was parallel to the dilution rate down to a value as low as 0.3 d-1. For both NS0 cell lines, the G1 cell population decreased, while the S and G2/M cell populations increased as the dilution rate was reduced. The antibody titer of the control cells increased from 7 to 21 μg·ml-1 as the dilution rate was reduced from 0.8 to 0.2 d-1. With an initial increase from 2 to 15 μg·ml-1 as the dilution rate was reduced from 0.8 to 0.4 d-1, the antibody titer of the Bcl-2 cells remained constant as the dilution rate was further reduced to 0.2 d-1. A good correlation between specific antibody production rate and the percentage of G2/M cells was observed. © 2005, The Society for Biotechnology.