Spectroscopic and microscopic evidence for adsorption of lipase from Candida rugosa onto calcined layered double hydroxides
Lipase from Candida rugosa (CRL) was adsorbed onto three calcined layered double hydroxide (CLDH): Mg/A1-NO3- (CMAN) and Zn/A1-NO3- (CZAN). Scanning Electron Microscopy-Energy Dispersive X-ray Spectroscopy (SEM-EDX) and protein staining light microscopy analyses were applied to provide direct spect...
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| Main Authors: | , , , , , |
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| Format: | Conference or Workshop Item |
| Language: | English |
| Published: |
2007
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| Online Access: | http://psasir.upm.edu.my/id/eprint/18994/1/ID%2018994.pdf http://psasir.upm.edu.my/id/eprint/18994/ |
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| Institution: | Universiti Putra Malaysia |
| Language: | English |
| Summary: | Lipase from Candida rugosa (CRL) was adsorbed onto three calcined layered double hydroxide (CLDH): Mg/A1-NO3- (CMAN) and Zn/A1-NO3- (CZAN). Scanning Electron Microscopy-Energy Dispersive X-ray Spectroscopy (SEM-EDX) and protein staining light microscopy analyses were applied to provide direct spectroscopic and microscopic evidence for the adsorption of CRL onto CLDH. SEM-EDX spectra show CRL presence in CMAN-CRL, CNAN-CRL and CZAN-CRL prepared. Blue spots on the surface of CMAN-CRL,CNAN-CRL and CZAN-CRL stained with Coomasie Blue R confirm protein adsorption onto supports. The catalytic activity of all three CLDH-CRL was also evaluated in synthesis of methyl caprylate and results showed that CMAN-CRL and CNAN-CRL exhibited 70% conversion while CZAN-CRL exhibited 80% conversion compared to free lipase that exhibited only 6% conversion only. |
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