Spectroscopic and microscopic evidence for adsorption of lipase from Candida rugosa onto calcined layered double hydroxides

• Main Authors: Md. Yunus, Noor Mona, Abdul Rahman, Mohd Basyaruddin, Basri, Mahiran, Hussein, Mohd Zobir, Salleh, Abu Bakar, Raja Abdul Rahman, Raja Noor Zaliha
• Format: Conference or Workshop Item
• Language: English
• Published: 2007
• Online Access: http://psasir.upm.edu.my/id/eprint/18994/1/ID%2018994.pdf; http://psasir.upm.edu.my/id/eprint/18994/
• Institution: Universiti Putra Malaysia

Lipase from Candida rugosa (CRL) was adsorbed onto three calcined layered double hydroxide (CLDH): Mg/A1-NO3- (CMAN) and Zn/A1-NO3- (CZAN). Scanning Electron Microscopy-Energy Dispersive X-ray Spectroscopy (SEM-EDX) and protein staining light microscopy analyses were applied to provide direct spectroscopic and microscopic evidence for the adsorption of CRL onto CLDH. SEM-EDX spectra show CRL presence in CMAN-CRL, CNAN-CRL and CZAN-CRL prepared. Blue spots on the surface of CMAN-CRL,CNAN-CRL and CZAN-CRL stained with Coomasie Blue R confirm protein adsorption onto supports. The catalytic activity of all three CLDH-CRL was also evaluated in synthesis of methyl caprylate and results showed that CMAN-CRL and CNAN-CRL exhibited 70% conversion while CZAN-CRL exhibited 80% conversion compared to free lipase that exhibited only 6% conversion only.