Angiotensin II stimulates expression of transcription factors c-Jun and c-Fos in cyclosporine induced human gingival fibroblasts

• Main Authors: Subramani, Tamilselvan, Rao, Suresh, Senthilkumar, Kamatchiammal, Perisamy, Saundararajan, Mohamed Alitheen, Noorjahan Banu
• Format: Article
• Language: English
• Published: Centro Regional de Investigaciones Cientificas y Tecnologicas 2014
• Online Access: http://psasir.upm.edu.my/id/eprint/36461/1/Angiotensin%20II%20stimulates%20expression%20of%20transcription%20factors%20c.pdf; http://psasir.upm.edu.my/id/eprint/36461/; http://www.cricyt.edu.ar/biocell/vol/37_3.html
• Institution: Universiti Putra Malaysia

The present study demonstrates that the expression of c-Jun and c-Fos are elevated in gingival fibro- blast cells treated with angiotensin II and cyclosporine. The healthy human gingival tissues were collected and gingival fibroblasts were isolated and cultured. We used RT-PCR and Western blot analysis to identify the expression of c-Jun and c-Fos in cyclosporine and angiotensin II treated human gingival fibroblast cells. We found that angiotensin II in combination with cyclosporine induces c-Jun and c-Fos expressions significantly; however, the angiotensin II antagonist losartan inhibits the expression of c-Jun and c-Fos (p < 0.01). The data suggest that angiotensin II in combination with cyclosporine modulates the expression of c-Jun and c-Fos in human gingival fibroblast cells.