GC-MS-based metabolite profiling of Cosmos caudatus leaves possessing alpha-glucosidase inhibitory activity

• Main Authors: Javadi, Neda, Abas, Faridah, Abdul Hamid, Azizah, Simoh, Sanimah, Shaari, Khozirah, Ismail, Intan Safinar, Mediani, Ahmed, Khatib, Alfi
• Format: Article
• Language: English
• Published: Institute of Food Technologists 2014
• Online Access: http://psasir.upm.edu.my/id/eprint/37740/1/37740.pdf; http://psasir.upm.edu.my/id/eprint/37740/; http://onlinelibrary.wiley.com/doi/10.1111/1750-3841.12491/abstract
• Institution: Universiti Putra Malaysia

Cosmos caudatus, which is known as “Ulam Raja,” is an herbal plant used in Malaysia to enhance vitality. This study focused on the evaluation of the α-glucosidase inhibitory activity of different ethanolic extracts of C. caudatus. Six series of samples extracted with water, 20%, 40%, 60%, 80%, and 100% ethanol (EtOH) were employed. Gas chromatography-mass spectrometry (GC-MS) and orthogonal partial least-squares (OPLS) analysis was used to correlate bioactivity of different extracts to different metabolite profiles of C. caudatus. The obtained OPLS scores indicated a distinct and remarkable separation into 6 clusters, which were indicative of the 6 different ethanol concentrations. GC-MS can be integrated with multivariate data analysis to identify compounds that inhibit α-glucosidase activity. In addition, catechin, α-linolenic acid, α-D-glucopyranoside, and vitamin E compounds were identified and indicate the potential α-glucosidase inhibitory activity of this herb.