Lipase-catalysed production and chemical composition of diacylglycerols from soybean oil deodoriser distillate
Diacylglycerols (DAG) were enzymatically produced by lipase-catalysed esterification of glycerol with fatty acids from soybean oil deodoriser distillate (SODD). Effects of reaction parameters such as reaction time, temperature, enzyme type, enzyme load, substrate molar ratio and water content, as we...
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2004
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my.upm.eprints.72892015-10-06T00:47:47Z http://psasir.upm.edu.my/id/eprint/7289/ Lipase-catalysed production and chemical composition of diacylglycerols from soybean oil deodoriser distillate Lo, Seong Koon Baharin, Badlishah Sham Tan, Chin Ping Lai, Oi Ming Diacylglycerols (DAG) were enzymatically produced by lipase-catalysed esterification of glycerol with fatty acids from soybean oil deodoriser distillate (SODD). Effects of reaction parameters such as reaction time, temperature, enzyme type, enzyme load, substrate molar ratio and water content, as well as the effect of molecular sieves as water adsorbent were studied. Lipozyme RM IM was determined to be the most effective among the lipases screened. The following conditions yielded 69.9% DAG (all percentages are wt/wt): 4 h reaction time, 65 °C reaction temperature, 10% Lipozyme RM IM, 2.5:1 fatty acid to glycerol molar ratio, and 30% molecular sieves. DAG synthesis of 11.9% was still observed at 10% water content. After purification, the product oil contained 86.3% DAG. This oil consisted predominantly of 1,3-diolein (19.1%), 1-oleoyl-3-linoleoyl-glycerol (18.2%) and 1-oleoyl-2-linoleoyl-glycerol (16.6%). The fatty acid profile of the oil was similar to that of refined, bleached and deodorised (RBD) soybean oil. The % ratio of 1,3- to 1,2-positional isomers of DAG was at 56:44. Wiley-Blackwell 2004-04-21 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/7289/1/Lipase-catalysed%20production%20and%20chemical%20composition%20of%20diacylglycerols%20from%20soybean%20oil%20deodoriser%20distillate.pdf Lo, Seong Koon and Baharin, Badlishah Sham and Tan, Chin Ping and Lai, Oi Ming (2004) Lipase-catalysed production and chemical composition of diacylglycerols from soybean oil deodoriser distillate. European Journal of Lipid Science and Technology, 106 (4). pp. 218-224. ISSN 1438-7697 http://dx.doi.org/10.1002/ejlt.200300888 10.1002/ejlt.200300888 English |
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Diacylglycerols (DAG) were enzymatically produced by lipase-catalysed esterification of glycerol with fatty acids from soybean oil deodoriser distillate (SODD). Effects of reaction parameters such as reaction time, temperature, enzyme type, enzyme load, substrate molar ratio and water content, as well as the effect of molecular sieves as water adsorbent were studied. Lipozyme RM IM was determined to be the most effective among the lipases screened. The following conditions yielded 69.9% DAG (all percentages are wt/wt): 4 h reaction time, 65 °C reaction temperature, 10% Lipozyme RM IM, 2.5:1 fatty acid to glycerol molar ratio, and 30% molecular sieves. DAG synthesis of 11.9% was still observed at 10% water content. After purification, the product oil contained 86.3% DAG. This oil consisted predominantly of 1,3-diolein (19.1%), 1-oleoyl-3-linoleoyl-glycerol (18.2%) and 1-oleoyl-2-linoleoyl-glycerol (16.6%). The fatty acid profile of the oil was similar to that of refined, bleached and deodorised (RBD) soybean oil. The % ratio of 1,3- to 1,2-positional isomers of DAG was at 56:44. |
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Article |
author |
Lo, Seong Koon Baharin, Badlishah Sham Tan, Chin Ping Lai, Oi Ming |
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Lo, Seong Koon Baharin, Badlishah Sham Tan, Chin Ping Lai, Oi Ming Lipase-catalysed production and chemical composition of diacylglycerols from soybean oil deodoriser distillate |
author_facet |
Lo, Seong Koon Baharin, Badlishah Sham Tan, Chin Ping Lai, Oi Ming |
author_sort |
Lo, Seong Koon |
title |
Lipase-catalysed production and chemical composition of diacylglycerols from soybean oil deodoriser distillate |
title_short |
Lipase-catalysed production and chemical composition of diacylglycerols from soybean oil deodoriser distillate |
title_full |
Lipase-catalysed production and chemical composition of diacylglycerols from soybean oil deodoriser distillate |
title_fullStr |
Lipase-catalysed production and chemical composition of diacylglycerols from soybean oil deodoriser distillate |
title_full_unstemmed |
Lipase-catalysed production and chemical composition of diacylglycerols from soybean oil deodoriser distillate |
title_sort |
lipase-catalysed production and chemical composition of diacylglycerols from soybean oil deodoriser distillate |
publisher |
Wiley-Blackwell |
publishDate |
2004 |
url |
http://psasir.upm.edu.my/id/eprint/7289/1/Lipase-catalysed%20production%20and%20chemical%20composition%20of%20diacylglycerols%20from%20soybean%20oil%20deodoriser%20distillate.pdf http://psasir.upm.edu.my/id/eprint/7289/ http://dx.doi.org/10.1002/ejlt.200300888 |
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