Dengue E protein detection using a graphene oxide integrated tapered optical fiber sensor

• Main Authors: Abu Bakar, Muhammad Hafiz, Mahdi, Mohd Adzir, Yaacob, Mohd Hanif, Amir Hamzah, Amir Syahir, Lim, Hong Ngee, Mustapha Kamil, Yasmin
• Format: Article
• Language: English
• Published: Institute of Electrical and Electronics Engineers 2019
• Online Access: http://psasir.upm.edu.my/id/eprint/81089/1/EPROTEIN.pdf; http://psasir.upm.edu.my/id/eprint/81089/; https://ieeexplore.ieee.org/document/8384018
• Institution: Universiti Putra Malaysia

We report the use of a biofunctionalized tapered optical fiber based sensor with the integration of graphene oxide (GO) for the detection of Dengue virus (DENV) II E proteins. The tapered region was deposited with GO and functionalized with anti-DENV II E protein IgG antibodies to be tested with different concentrations of DENV II E proteins. The sensor obtained a detection limit of 1 pM with a sensitivity value of 12.77 nm/nM, which was better compared to previously reported studies. The sensor also showed high precision, great selectivity, and high affinity toward E protein with a dissociation constant of Kd = 1.11 × 10–9 M–1. The proposed sensor has undoubtedly exhibited the immense potential of nanomaterial integration for future advancements of dengue diagnostics.