Fractionation of tilapia by-product protein hydrolysate using multilayer configuration of ultrafiltration membrane
Production of small-sized peptides is significant because of their health benefits. Ultrafiltration (UF) membrane provides an effective fractionation of small-sized peptides on a large scale. Thus, the present study was aimed to evaluate the performance of multilayer UF membrane in fractionating til...
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Multidisciplinary Digital Publishing Institute
2021
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my.upm.eprints.973902022-07-27T08:47:06Z http://psasir.upm.edu.my/id/eprint/97390/ Fractionation of tilapia by-product protein hydrolysate using multilayer configuration of ultrafiltration membrane Roslan, Jumardi Mustapa Kamal, Siti Mazlina Md. Yunos, Khairul Faezah Abdullah, Norhafizah Production of small-sized peptides is significant because of their health benefits. Ultrafiltration (UF) membrane provides an effective fractionation of small-sized peptides on a large scale. Thus, the present study was aimed to evaluate the performance of multilayer UF membrane in fractionating tilapia fish by-product (TB) protein hydrolysate by observing the permeate flux, peptide transmission, and peptide distribution under different stirring speed, pH of feed solution, and salt concentration (NaCl). The fractionation process was carried out using a dead-end UF membrane system that consists of a stack of two membrane sheets with different (10/5 kDa) and similar (5/5 kDa) pore sizes in one device. The highest permeate flux (10/5 kDa–39.5 to 47.3 L/m2.h; 5/5 kDa– 15.8 to 20.3 L/m2.h) and peptide transmission (10/5 kDa–51.8 to 61.0%; 5/5 kDa–18.3 to 23.3%) for both multilayer membrane configurations were obtained at 3.0 bar, 600 rpm, pH 8, and without the addition of salt. It was also found that the permeates were enriched with small-size peptides (<500 Da) with a concentration of 0.58 g/L (10/5 kDa) and 0.65 g/L (5/5 kDa) as compared to large-sized peptides (500–1500 Da) with concentration of 0.56 g/L (10/5 kDa) and 0.36 g/L (5/5 kDa). This might indicate the enrichment of small-size peptides through the multilayer membrane which could potentially enhance the biological activity of the protein hydrolysate fraction. Multidisciplinary Digital Publishing Institute 2021 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/97390/1/ABSTRACT.pdf Roslan, Jumardi and Mustapa Kamal, Siti Mazlina and Md. Yunos, Khairul Faezah and Abdullah, Norhafizah (2021) Fractionation of tilapia by-product protein hydrolysate using multilayer configuration of ultrafiltration membrane. Processes, 9 (3). art. no. 446. pp. 1-15. ISSN 2227-9717 https://www.mdpi.com/2227-9717/9/3/446 10.3390/pr9030446 |
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Production of small-sized peptides is significant because of their health benefits. Ultrafiltration (UF) membrane provides an effective fractionation of small-sized peptides on a large scale. Thus, the present study was aimed to evaluate the performance of multilayer UF membrane in fractionating tilapia fish by-product (TB) protein hydrolysate by observing the permeate flux, peptide transmission, and peptide distribution under different stirring speed, pH of feed solution, and salt concentration (NaCl). The fractionation process was carried out using a dead-end UF membrane system that consists of a stack of two membrane sheets with different (10/5 kDa) and similar (5/5 kDa) pore sizes in one device. The highest permeate flux (10/5 kDa–39.5 to 47.3 L/m2.h; 5/5 kDa– 15.8 to 20.3 L/m2.h) and peptide transmission (10/5 kDa–51.8 to 61.0%; 5/5 kDa–18.3 to 23.3%) for both multilayer membrane configurations were obtained at 3.0 bar, 600 rpm, pH 8, and without the addition of salt. It was also found that the permeates were enriched with small-size peptides (<500 Da) with a concentration of 0.58 g/L (10/5 kDa) and 0.65 g/L (5/5 kDa) as compared to large-sized peptides (500–1500 Da) with concentration of 0.56 g/L (10/5 kDa) and 0.36 g/L (5/5 kDa). This might indicate the enrichment of small-size peptides through the multilayer membrane which could potentially enhance the biological activity of the protein hydrolysate fraction. |
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Article |
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Roslan, Jumardi Mustapa Kamal, Siti Mazlina Md. Yunos, Khairul Faezah Abdullah, Norhafizah |
spellingShingle |
Roslan, Jumardi Mustapa Kamal, Siti Mazlina Md. Yunos, Khairul Faezah Abdullah, Norhafizah Fractionation of tilapia by-product protein hydrolysate using multilayer configuration of ultrafiltration membrane |
author_facet |
Roslan, Jumardi Mustapa Kamal, Siti Mazlina Md. Yunos, Khairul Faezah Abdullah, Norhafizah |
author_sort |
Roslan, Jumardi |
title |
Fractionation of tilapia by-product protein hydrolysate using multilayer configuration of ultrafiltration membrane |
title_short |
Fractionation of tilapia by-product protein hydrolysate using multilayer configuration of ultrafiltration membrane |
title_full |
Fractionation of tilapia by-product protein hydrolysate using multilayer configuration of ultrafiltration membrane |
title_fullStr |
Fractionation of tilapia by-product protein hydrolysate using multilayer configuration of ultrafiltration membrane |
title_full_unstemmed |
Fractionation of tilapia by-product protein hydrolysate using multilayer configuration of ultrafiltration membrane |
title_sort |
fractionation of tilapia by-product protein hydrolysate using multilayer configuration of ultrafiltration membrane |
publisher |
Multidisciplinary Digital Publishing Institute |
publishDate |
2021 |
url |
http://psasir.upm.edu.my/id/eprint/97390/1/ABSTRACT.pdf http://psasir.upm.edu.my/id/eprint/97390/ https://www.mdpi.com/2227-9717/9/3/446 |
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