Analysis Of Proteins Extracted From The Leaves Of Piper Sarmentosum

A gel based proteomic method has been developed for the leaf tissues of P. sarmentosum. A suitable protein extraction method termed as total protein extraction followed by trichloroacetic acid (TCA)/acetone precipitation was applied and has been shown to produce good leaf tissue extract that was com...

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Main Author:	Shim , Siang yian
Format:	Thesis
Language:	English
Published:	2010
Subjects:	TP1-1185 Chemical technology
Online Access:	http://eprints.usm.my/42578/1/SHIM_SIANG_YIAN.pdf http://eprints.usm.my/42578/
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Institution:	Universiti Sains Malaysia
Language:	English

id	my.usm.eprints.42578
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spelling	my.usm.eprints.42578 http://eprints.usm.my/42578/ Analysis Of Proteins Extracted From The Leaves Of Piper Sarmentosum Shim , Siang yian TP1-1185 Chemical technology A gel based proteomic method has been developed for the leaf tissues of P. sarmentosum. A suitable protein extraction method termed as total protein extraction followed by trichloroacetic acid (TCA)/acetone precipitation was applied and has been shown to produce good leaf tissue extract that was compatible with 2-DE separation. The protein profiling of leaf tissues of P. sarmentosum showed a total of 242 protein spots and from which 283 proteins were identified by LCMS/MS analysis. Most of these identified proteins were involved in metabolism (including photosynthesis), protein destination and storage, energy and transport, and defense and stress related protein. 2010-10 Thesis NonPeerReviewed application/pdf en http://eprints.usm.my/42578/1/SHIM_SIANG_YIAN.pdf Shim , Siang yian (2010) Analysis Of Proteins Extracted From The Leaves Of Piper Sarmentosum. Masters thesis, Universiti Sains Malaysia.
institution	Universiti Sains Malaysia
building	Hamzah Sendut Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Sains Malaysia
content_source	USM Institutional Repository
url_provider	http://eprints.usm.my/
language	English
topic	TP1-1185 Chemical technology
spellingShingle	TP1-1185 Chemical technology Shim , Siang yian Analysis Of Proteins Extracted From The Leaves Of Piper Sarmentosum
description	A gel based proteomic method has been developed for the leaf tissues of P. sarmentosum. A suitable protein extraction method termed as total protein extraction followed by trichloroacetic acid (TCA)/acetone precipitation was applied and has been shown to produce good leaf tissue extract that was compatible with 2-DE separation. The protein profiling of leaf tissues of P. sarmentosum showed a total of 242 protein spots and from which 283 proteins were identified by LCMS/MS analysis. Most of these identified proteins were involved in metabolism (including photosynthesis), protein destination and storage, energy and transport, and defense and stress related protein.
format	Thesis
author	Shim , Siang yian
author_facet	Shim , Siang yian
author_sort	Shim , Siang yian
title	Analysis Of Proteins Extracted From The Leaves Of Piper Sarmentosum
title_short	Analysis Of Proteins Extracted From The Leaves Of Piper Sarmentosum
title_full	Analysis Of Proteins Extracted From The Leaves Of Piper Sarmentosum
title_fullStr	Analysis Of Proteins Extracted From The Leaves Of Piper Sarmentosum
title_full_unstemmed	Analysis Of Proteins Extracted From The Leaves Of Piper Sarmentosum
title_sort	analysis of proteins extracted from the leaves of piper sarmentosum
publishDate	2010
url	http://eprints.usm.my/42578/1/SHIM_SIANG_YIAN.pdf http://eprints.usm.my/42578/
_version_	1643710528791511040

Analysis Of Proteins Extracted From The Leaves Of Piper Sarmentosum

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