Cloning, extracellular expression and characterization of a predominant beta-CGTase from Bacillus sp G1 in E-coli

Cloning, extracellular expression and characterization of a predominant beta-CGTase from Bacillus sp G1 in E-coli

The cyclodextrin glucanotransferase (CGTase, EC 2.4.1.19) gene from Bacillus sp. G1 was successfully isolated and cloned into Escherichia coli. Analysis of the nucleotide sequence revealed the presence of an open reading frame of 2,109 bp and encoded a 674 amino acid protein. Purified CGTase exhibit...

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Bibliographic Details
Main Authors: Ong, Rui Min, Goh, Kian Mau, Mahadi, Nor Muhammad, Hassan, Osman, Raja Abdul Rahman, Raja Noor Zaliha, Md. Illias, Rosli
Format: Article
Published: Springer Verlag 2008
Subjects:
Q Science (General)
Online Access:http://eprints.utm.my/id/eprint/12511/
http://www.springerlink.com/content/u165261504231305/
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Institution: Universiti Teknologi Malaysia

Internet

http://eprints.utm.my/id/eprint/12511/
http://www.springerlink.com/content/u165261504231305/

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