Cloning, extracellular expression and characterization of a predominant beta-CGTase from Bacillus sp G1 in E-coli

• Main Authors: Ong, Rui Min, Goh, Kian Mau, Mahadi, Nor Muhammad, Hassan, Osman, Raja Abdul Rahman, Raja Noor Zaliha, Md. Illias, Rosli
• Format: Article
• Published: Springer Verlag 2008
• Subjects: Q Science (General)
• Online Access: http://eprints.utm.my/id/eprint/12511/; http://www.springerlink.com/content/u165261504231305/
• Institution: Universiti Teknologi Malaysia

The cyclodextrin glucanotransferase (CGTase, EC 2.4.1.19) gene from Bacillus sp. G1 was successfully isolated and cloned into Escherichia coli. Analysis of the nucleotide sequence revealed the presence of an open reading frame of 2,109 bp and encoded a 674 amino acid protein. Purified CGTase exhibit...