Apparent molal volume and compressibility of glucose and maltose at different temperatures in lysozyme solution

In the present research, we study the thermody-namic parameters of ternary system of two different sug-ars, i.e., (a)D(-)glucose+lysozyme+water and (b) maltose+lysozyme+water. It has been done at various temperatures(293.15, 303.15, 313.15 and 323.15K) and concentrations ofsugars. The apparent molal...

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Bibliographic Details
Main Authors: Siddique, Jamal Akhter, Sharma, Sheetal, Naqvi, Saeeda, Ahmad, Akil, Khatoon, Asma, Mohd. Setapar, Siti Hamidah
Format: Article
Published: Springer Verlag 2015
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Online Access:http://eprints.utm.my/id/eprint/57848/
http://dx.doi.org/10.1007/s13369-015-1818-3
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Institution: Universiti Teknologi Malaysia
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Summary:In the present research, we study the thermody-namic parameters of ternary system of two different sug-ars, i.e., (a)D(-)glucose+lysozyme+water and (b) maltose+lysozyme+water. It has been done at various temperatures(293.15, 303.15, 313.15 and 323.15K) and concentrations ofsugars. The apparent molal volume and apparent molal com-pressibilities have been calculated for these systems. Thepartial molal volume ofD(-)glucose and maltose has alsobeen evaluated in aqueous lysozyme solution. We have madean attempt to examine transfer volume of the said solutesfrom water to aqueous lysozyme solution. Interaction be-tween sugars and water molecules is expected to create polarenvironment, which helps to increase the hydrophobic inter-actions in proteins, thus stabilizing the protein.