Determination of tamoxifen and its metabolites using micelle to solvent stacking in nonaqueous capillary electrophoresis
Micelle to solvent stacking was implemented for the recently established NACE-C4D method to determine tamoxifen and its metabolites in standard samples and human plasma of breast cancer patients. For stacking, the standard samples and extract after liquid-liquid extraction (LLE) were prepared in met...
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| Main Authors: | , , |
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| Format: | Article |
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Wiley-VCH Verlag
2016
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| Subjects: | |
| Online Access: | http://eprints.utm.my/id/eprint/69173/ http://dx.doi.org/10.1002/elps.201600010 |
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| Institution: | Universiti Teknologi Malaysia |
| Summary: | Micelle to solvent stacking was implemented for the recently established NACE-C4D method to determine tamoxifen and its metabolites in standard samples and human plasma of breast cancer patients. For stacking, the standard samples and extract after liquid-liquid extraction (LLE) were prepared in methanol and the resulting sample solution was pressure injected after a micellar plug of SDS. Factors that affected the stacking such as SDS concentration, micelle, and sample plug length were examined. The sensitivity enhancement factor (peak height from stacking/peak height from typical injection of sample in BGE) was 15-22. The method detection limits with LLE were in the range of 5-10 ng/mL, which was lower than the established method (where the LLE extract was also prepared in methanol) with reported method detection limits of 25-40 ng/mL. The intraday and interday repeatability were in the range of 1.0-3.4% and 3.8-6.5%, respectively. |
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