Cloning, extracellular expression and characterization of a predominant β-CGTase from Bacillus sp. G1 in E. coli

Cloning, extracellular expression and characterization of a predominant β-CGTase from Bacillus sp. G1 in E. coli

The cyclodextrin glucanotransferase (CGTase, EC 2.4.1.19) gene from Bacillus sp. G1 was successfully isolated and cloned into Escherichia coli. Analysis of the nucleotide sequence revealed the presence of an open reading frame of 2,109 bp and encoded a 674 amino acid protein. Purified CGTase exhibit...

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Bibliographic Details
Main Authors: Ong, Rui Min, Goh, Kian Mau, Mahadi, Nor Muhammad, Hassan, Osman, Rahman, Raja Noor Zaliha Raja Abdul, Illias, Rosli Md.
Format: Article
Published: Springer Berlin / Heidelberg 2008
Subjects:
TP Chemical technology
Online Access:http://eprints.utm.my/id/eprint/7263/
http://dx.doi.org/10.1007/s10295-008-0462-2
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Institution: Universiti Teknologi Malaysia

Internet

http://eprints.utm.my/id/eprint/7263/
http://dx.doi.org/10.1007/s10295-008-0462-2

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