Cloning, extracellular expression and characterization of a predominant β-CGTase from Bacillus sp. G1 in E. coli

• Main Authors: Ong, Rui Min, Goh, Kian Mau, Mahadi, Nor Muhammad, Hassan, Osman, Rahman, Raja Noor Zaliha Raja Abdul, Illias, Rosli Md.
• Format: Article
• Published: Springer Berlin / Heidelberg 2008
• Subjects: TP Chemical technology
• Online Access: http://eprints.utm.my/id/eprint/7263/; http://dx.doi.org/10.1007/s10295-008-0462-2
• Institution: Universiti Teknologi Malaysia

The cyclodextrin glucanotransferase (CGTase, EC 2.4.1.19) gene from Bacillus sp. G1 was successfully isolated and cloned into Escherichia coli. Analysis of the nucleotide sequence revealed the presence of an open reading frame of 2,109 bp and encoded a 674 amino acid protein. Purified CGTase exhibit...