Biodegradation of pyrene by Candida sp. S1 under high salinity conditions
Polycyclic aromatic hydrocarbon is a toxic recalcitrant environmental pollutant and its removal from the environment is very essential. In this study, a novel S1 strain isolated from the tropical rain forest was identified as Candida species based on 18S rRNA. The pyrene biodegradation was performed...
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| Main Authors: | , , , |
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| Format: | Article |
| Published: |
Springer Verlag
2017
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| Subjects: | |
| Online Access: | http://eprints.utm.my/id/eprint/75442/ https://www.scopus.com/inward/record.uri?eid=2-s2.0-85020694592&doi=10.1007%2fs00449-017-1798-7&partnerID=40&md5=38fcb6afce50139d5f2700be7e6a1116 |
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| Institution: | Universiti Teknologi Malaysia |
| Summary: | Polycyclic aromatic hydrocarbon is a toxic recalcitrant environmental pollutant and its removal from the environment is very essential. In this study, a novel S1 strain isolated from the tropical rain forest was identified as Candida species based on 18S rRNA. The pyrene biodegradation was performed by Candida sp. S1. Pyrene was 35% degraded in 15 days. The percentage of pyrene biodegradation increased up to 75% with 24 g L−1 of sodium chloride and decreased along with increasing salinity. Under the acidic condition, the biodegradation was increased up to 60% at pH 5. It was also found that the increasing glucose concentration of more than 10 g L−1 had no significant effect on pyrene biodegradation, while agitation proved to have greater influence. There was a positive relationship between biomass growth and biodegradation rate of pyrene. One pyrene metabolite was identified from the extract solution and analyzed by a thin-layer chromatography, UV–visible absorption and gas chromatography–mass spectrometry. The metabolite found in the pyrene degradation was benzoic acid. Suitable conditions must be found to promote a successful microbial augmentation in liquid culture. |
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