Biodegradation of pyrene by Candida sp. S1 under high salinity conditions

• Main Authors: Hadibarata, T., Khudhair, A. B., Kristanti, R. A., Kamyab, H.
• Format: Article
• Published: Springer Verlag 2017
• Subjects: TA Engineering (General). Civil engineering (General)
• Online Access: http://eprints.utm.my/id/eprint/75442/; https://www.scopus.com/inward/record.uri?eid=2-s2.0-85020694592&doi=10.1007%2fs00449-017-1798-7&partnerID=40&md5=38fcb6afce50139d5f2700be7e6a1116
• Institution: Universiti Teknologi Malaysia

Polycyclic aromatic hydrocarbon is a toxic recalcitrant environmental pollutant and its removal from the environment is very essential. In this study, a novel S1 strain isolated from the tropical rain forest was identified as Candida species based on 18S rRNA. The pyrene biodegradation was performed by Candida sp. S1. Pyrene was 35% degraded in 15 days. The percentage of pyrene biodegradation increased up to 75% with 24 g L−1 of sodium chloride and decreased along with increasing salinity. Under the acidic condition, the biodegradation was increased up to 60% at pH 5. It was also found that the increasing glucose concentration of more than 10 g L−1 had no significant effect on pyrene biodegradation, while agitation proved to have greater influence. There was a positive relationship between biomass growth and biodegradation rate of pyrene. One pyrene metabolite was identified from the extract solution and analyzed by a thin-layer chromatography, UV–visible absorption and gas chromatography–mass spectrometry. The metabolite found in the pyrene degradation was benzoic acid. Suitable conditions must be found to promote a successful microbial augmentation in liquid culture.