Optimisation of growth medium for the production of cyclodextrin glucanotransferase from Bacillus stearothermophilus HR1 using response surface methodology

• Main Authors: Abd. Rahman, Roshanida, Md. Illias, Rosli, Mohd Ghazali, Mohd Nawawi, Ismail, Ahmad Fauzi, Hassan, Osman, Kamaruddin, Kamarulzaman
• Format: Article
• Published: Elsevier Science B.V., Amsterdam 2004
• Subjects: TP Chemical technology
• Online Access: http://eprints.utm.my/id/eprint/772/; http://dx.doi.org/10.1016/j.procbio.2003.10.014
• Institution: Universiti Teknologi Malaysia

Cyclodextrin glucanotransferase (CGTase) activity was observed when the bacterium was grown in the medium at various initial pH values, containing carbon, nitrogen, phosphorus and mineral salt sources at 50 oC for 24 h in the shake flasks. The optimisation of this growth medium was carried out using...