Optimisation of growth medium for the production of cyclodextrin glucanotransferase from Bacillus stearothermophilus HR1 using response surface methodology

Cyclodextrin glucanotransferase (CGTase) activity was observed when the bacterium was grown in the medium at various initial pH values, containing carbon, nitrogen, phosphorus and mineral salt sources at 50 oC for 24 h in the shake flasks. The optimisation of this growth medium was carried out using...

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Main Authors: Abd. Rahman, Roshanida, Md. Illias, Rosli, Mohd Ghazali, Mohd Nawawi, Ismail, Ahmad Fauzi, Hassan, Osman, Kamaruddin, Kamarulzaman
Format: Article
Published: Elsevier Science B.V., Amsterdam 2004
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Online Access:http://eprints.utm.my/id/eprint/772/
http://dx.doi.org/10.1016/j.procbio.2003.10.014
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spelling my.utm.7722017-03-06T08:08:03Z http://eprints.utm.my/id/eprint/772/ Optimisation of growth medium for the production of cyclodextrin glucanotransferase from Bacillus stearothermophilus HR1 using response surface methodology Abd. Rahman, Roshanida Md. Illias, Rosli Mohd Ghazali, Mohd Nawawi Ismail, Ahmad Fauzi Hassan, Osman Kamaruddin, Kamarulzaman TP Chemical technology Cyclodextrin glucanotransferase (CGTase) activity was observed when the bacterium was grown in the medium at various initial pH values, containing carbon, nitrogen, phosphorus and mineral salt sources at 50 oC for 24 h in the shake flasks. The optimisation of this growth medium was carried out using response surface methodology. The design contains a total of 32 experimental trials involving 10 star points and 6 replicates at the centre points. The design was employed by selecting sago starch, peptone from casein, K2HPO4, CaCl2 and initial pH as five independent variables in this study. The optimal calculated values of tested variables for maximal production of CGTase were found to be comprised of: sago starch, 16.02 g/l; peptone from casein, 20 g/l; K2HPO4, 1.4 g/l; CaCl2, 0.2 g/l and initial pH, 7.54 with a predicted CGTase activity of 14.20 U/ml. These predicted optimal parameters were tested in the laboratory and the final CGTase activity obtained was very close to the predicted value at 14.80 U/ml. Elsevier Science B.V., Amsterdam 2004 Article PeerReviewed Abd. Rahman, Roshanida and Md. Illias, Rosli and Mohd Ghazali, Mohd Nawawi and Ismail, Ahmad Fauzi and Hassan, Osman and Kamaruddin, Kamarulzaman (2004) Optimisation of growth medium for the production of cyclodextrin glucanotransferase from Bacillus stearothermophilus HR1 using response surface methodology. Process Biochemistry, 39 . pp. 2053-2060. ISSN 0032-9592 http://dx.doi.org/10.1016/j.procbio.2003.10.014 10.1016/j.procbio.2003.10.014
institution Universiti Teknologi Malaysia
building UTM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Malaysia
content_source UTM Institutional Repository
url_provider http://eprints.utm.my/
topic TP Chemical technology
spellingShingle TP Chemical technology
Abd. Rahman, Roshanida
Md. Illias, Rosli
Mohd Ghazali, Mohd Nawawi
Ismail, Ahmad Fauzi
Hassan, Osman
Kamaruddin, Kamarulzaman
Optimisation of growth medium for the production of cyclodextrin glucanotransferase from Bacillus stearothermophilus HR1 using response surface methodology
description Cyclodextrin glucanotransferase (CGTase) activity was observed when the bacterium was grown in the medium at various initial pH values, containing carbon, nitrogen, phosphorus and mineral salt sources at 50 oC for 24 h in the shake flasks. The optimisation of this growth medium was carried out using response surface methodology. The design contains a total of 32 experimental trials involving 10 star points and 6 replicates at the centre points. The design was employed by selecting sago starch, peptone from casein, K2HPO4, CaCl2 and initial pH as five independent variables in this study. The optimal calculated values of tested variables for maximal production of CGTase were found to be comprised of: sago starch, 16.02 g/l; peptone from casein, 20 g/l; K2HPO4, 1.4 g/l; CaCl2, 0.2 g/l and initial pH, 7.54 with a predicted CGTase activity of 14.20 U/ml. These predicted optimal parameters were tested in the laboratory and the final CGTase activity obtained was very close to the predicted value at 14.80 U/ml.
format Article
author Abd. Rahman, Roshanida
Md. Illias, Rosli
Mohd Ghazali, Mohd Nawawi
Ismail, Ahmad Fauzi
Hassan, Osman
Kamaruddin, Kamarulzaman
author_facet Abd. Rahman, Roshanida
Md. Illias, Rosli
Mohd Ghazali, Mohd Nawawi
Ismail, Ahmad Fauzi
Hassan, Osman
Kamaruddin, Kamarulzaman
author_sort Abd. Rahman, Roshanida
title Optimisation of growth medium for the production of cyclodextrin glucanotransferase from Bacillus stearothermophilus HR1 using response surface methodology
title_short Optimisation of growth medium for the production of cyclodextrin glucanotransferase from Bacillus stearothermophilus HR1 using response surface methodology
title_full Optimisation of growth medium for the production of cyclodextrin glucanotransferase from Bacillus stearothermophilus HR1 using response surface methodology
title_fullStr Optimisation of growth medium for the production of cyclodextrin glucanotransferase from Bacillus stearothermophilus HR1 using response surface methodology
title_full_unstemmed Optimisation of growth medium for the production of cyclodextrin glucanotransferase from Bacillus stearothermophilus HR1 using response surface methodology
title_sort optimisation of growth medium for the production of cyclodextrin glucanotransferase from bacillus stearothermophilus hr1 using response surface methodology
publisher Elsevier Science B.V., Amsterdam
publishDate 2004
url http://eprints.utm.my/id/eprint/772/
http://dx.doi.org/10.1016/j.procbio.2003.10.014
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