Reduction of Extracellular Proteases Increased Activity and Stability of Heterologous Protein in Aspergillus niger

• Main Authors: Kamaruddin, Nurhaida, Storms, Reginald, Mahadi, Nor Muhammad, Md. Illias, Rosli, Abu Bakar, Farah Diba, Abdul Murad, Abdul Munir
• Format: Article
• Published: Springer Verlag 2018
• Subjects: TP Chemical technology
• Online Access: http://eprints.utm.my/id/eprint/84458/; http://dx.doi.org/10.1007/s13369-017-2914-3
• Institution: Universiti Teknologi Malaysia

The heterologous protein production in Aspergillus niger is often limited by the activity of the host extracellular proteases. To improve heterologous production, a transcription factor controlling expression of several extracellular protease-encoding genes, prtT, was deleted in A. niger PY11 and th...