Sequence analysis and comparative modelling of bromelain from pineapple
Protein structure can be determined by either experimental method or by computational prediction, which commonly known as comparative modelling. The objective of this study is to predict the structure of bromelain enzyme from pineapple using computational approaches. Results from BLAST showed that t...
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| Main Authors: | , , |
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| Format: | Conference or Workshop Item |
| Published: |
2020
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| Subjects: | |
| Online Access: | http://eprints.utm.my/id/eprint/90841/ http://dx.doi.org/10.1063/5.0027886 |
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| Institution: | Universiti Teknologi Malaysia |
| Summary: | Protein structure can be determined by either experimental method or by computational prediction, which commonly known as comparative modelling. The objective of this study is to predict the structure of bromelain enzyme from pineapple using computational approaches. Results from BLAST showed that the most suitable template for bromelain enzyme was procaricain with 40.58% of sequence identity determined by sequence alignment. MODELLER was used to predict the model using the method of satisfaction of spatial restraints. The quality of the predicted model was then analysed using Ramachandrans Plot and Verify3D Profile evaluation. The results suggest that the model is reliable and has good stereochemical properties. |
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