Ultrasensitive aptasensor using electrospun MXene/polyvinylidene fluoride nanofiber composite for Ochratoxin A detection

• Main Authors: Al-Dhahebi, A.M., Jose, R., Mustapha, M., Saheed, M.S.M.
• Format: Article
• Published: Elsevier Ltd 2022
• Online Access: https://www.scopus.com/inward/record.uri?eid=2-s2.0-85130371663&doi=10.1016%2fj.foodchem.2022.133105&partnerID=40&md5=86fc5c616235a8266264b126078d8dda; http://eprints.utp.edu.my/33011/
• Institution: Universiti Teknologi Petronas

Ochratoxin A (OTA) has harmful effects to human and animal health; therefore, sensitive and selective detection of OTA is highly demanded. Herein, an ultrasensitive electrochemical aptasensor electrode comprising electrospun MXene/polyvinylidene fluoride (Ti3C2Tx/PVDF) nanofiber composite is presented. Addition of Ti3C2Tx up to 13 effectively increased the fiber diameter and lowered the β-phase of PVDF nanofibers, consequently lowering the charge transfer resistance. The nanofiber composite is then coated on the screen-printed carbon electrode to chemically functionalized with saline and aldehyde groups for efficient aptamer loading. The optimized aptasensor demonstrated sensitive detection of OTA over the dynamic concentration range from 1 fg mL�1 to 1 ng mL�1 with a limit of detection of 2.15 fg mL�1 and quantification limit of 6.52 fg mL�1, with high selectivity. The aptasensor could detect the OTA at femtogram per milliliter concentration in grape juice samples, demonstrating its enormous potential for OTA detection in food industry. © 2022 Elsevier Ltd