Developmental time table of the sea urchin Diadema setosum found in Talin Bay Matuod, Lian, Batangas
Under controlled laboratory conditions of water temperature (25-26 centigrade) and salinity (30-34 ppt), the developmental time table of the sea urchin, Diadema setosum was established. Gametes were obtained via the chemical induction techniques by Kobayashi (1971). The sperm and eggs were mixed and...
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| Format: | text |
| Language: | English |
| Published: |
Animo Repository
1992
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| Online Access: | https://animorepository.dlsu.edu.ph/etd_bachelors/1100 |
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| Institution: | De La Salle University |
| Language: | English |
| Summary: | Under controlled laboratory conditions of water temperature (25-26 centigrade) and salinity (30-34 ppt), the developmental time table of the sea urchin, Diadema setosum was established. Gametes were obtained via the chemical induction techniques by Kobayashi (1971). The sperm and eggs were mixed and allowed to develop in culture beakers containing filtered aerated sea water. The developing embryos were observed microscopically (2150X) at different time points within the 48-hour culture period.The study showed that fertilization was attained 3-4 minutes after the sperms were added to the eggs. The 2-cell stage was observed 60-70 minutes, blastula stage recorded after 300 minutes and mid-gastrula 600 minutes after the sperms were added to the eggs. The 4-armed pluteal larvae stage was the highest developmental stage attained within 1860-1920 minutes. |
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