Developmental time table of the sea urchin Diadema setosum found in Talin Bay Matuod, Lian, Batangas

Under controlled laboratory conditions of water temperature (25-26 centigrade) and salinity (30-34 ppt), the developmental time table of the sea urchin, Diadema setosum was established. Gametes were obtained via the chemical induction techniques by Kobayashi (1971). The sperm and eggs were mixed and...

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Main Author: Perez, Marilou H.
Format: text
Language:English
Published: Animo Repository 1992
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Online Access:https://animorepository.dlsu.edu.ph/etd_bachelors/1100
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Institution: De La Salle University
Language: English
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spelling oai:animorepository.dlsu.edu.ph:etd_bachelors-21002021-06-21T03:21:31Z Developmental time table of the sea urchin Diadema setosum found in Talin Bay Matuod, Lian, Batangas Perez, Marilou H. Under controlled laboratory conditions of water temperature (25-26 centigrade) and salinity (30-34 ppt), the developmental time table of the sea urchin, Diadema setosum was established. Gametes were obtained via the chemical induction techniques by Kobayashi (1971). The sperm and eggs were mixed and allowed to develop in culture beakers containing filtered aerated sea water. The developing embryos were observed microscopically (2150X) at different time points within the 48-hour culture period.The study showed that fertilization was attained 3-4 minutes after the sperms were added to the eggs. The 2-cell stage was observed 60-70 minutes, blastula stage recorded after 300 minutes and mid-gastrula 600 minutes after the sperms were added to the eggs. The 4-armed pluteal larvae stage was the highest developmental stage attained within 1860-1920 minutes. 1992-01-01T08:00:00Z text https://animorepository.dlsu.edu.ph/etd_bachelors/1100 Bachelor's Theses English Animo Repository Sea-urchins Fertilization (Biology) Embryology Experimental
institution De La Salle University
building De La Salle University Library
continent Asia
country Philippines
Philippines
content_provider De La Salle University Library
collection DLSU Institutional Repository
language English
topic Sea-urchins
Fertilization (Biology)
Embryology
Experimental
spellingShingle Sea-urchins
Fertilization (Biology)
Embryology
Experimental
Perez, Marilou H.
Developmental time table of the sea urchin Diadema setosum found in Talin Bay Matuod, Lian, Batangas
description Under controlled laboratory conditions of water temperature (25-26 centigrade) and salinity (30-34 ppt), the developmental time table of the sea urchin, Diadema setosum was established. Gametes were obtained via the chemical induction techniques by Kobayashi (1971). The sperm and eggs were mixed and allowed to develop in culture beakers containing filtered aerated sea water. The developing embryos were observed microscopically (2150X) at different time points within the 48-hour culture period.The study showed that fertilization was attained 3-4 minutes after the sperms were added to the eggs. The 2-cell stage was observed 60-70 minutes, blastula stage recorded after 300 minutes and mid-gastrula 600 minutes after the sperms were added to the eggs. The 4-armed pluteal larvae stage was the highest developmental stage attained within 1860-1920 minutes.
format text
author Perez, Marilou H.
author_facet Perez, Marilou H.
author_sort Perez, Marilou H.
title Developmental time table of the sea urchin Diadema setosum found in Talin Bay Matuod, Lian, Batangas
title_short Developmental time table of the sea urchin Diadema setosum found in Talin Bay Matuod, Lian, Batangas
title_full Developmental time table of the sea urchin Diadema setosum found in Talin Bay Matuod, Lian, Batangas
title_fullStr Developmental time table of the sea urchin Diadema setosum found in Talin Bay Matuod, Lian, Batangas
title_full_unstemmed Developmental time table of the sea urchin Diadema setosum found in Talin Bay Matuod, Lian, Batangas
title_sort developmental time table of the sea urchin diadema setosum found in talin bay matuod, lian, batangas
publisher Animo Repository
publishDate 1992
url https://animorepository.dlsu.edu.ph/etd_bachelors/1100
_version_ 1712575659589500928