Isolation and screening of lactic acid-producing bacteria from buro (fermented rice with fish) for antimicrobial activity against multiple drug resistant bacteria

Lactic acid-producing bacteria (LAB) show great promise as potential alternative treatment for diseases caused by drug resistant pathogens. The study isolated LABs present in buro (fermented rice with fish) and screened them for inhibitory activities against multiple drug resistant (MDR) bacteria, n...

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Bibliographic Details
Main Author: Bantilan, Jan Lawrenz S.
Format: text
Language:English
Published: Animo Repository 2014
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Online Access:https://animorepository.dlsu.edu.ph/etd_bachelors/2787
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Institution: De La Salle University
Language: English
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Summary:Lactic acid-producing bacteria (LAB) show great promise as potential alternative treatment for diseases caused by drug resistant pathogens. The study isolated LABs present in buro (fermented rice with fish) and screened them for inhibitory activities against multiple drug resistant (MDR) bacteria, namely: extended spectrum β-lactamase-producing Escherichia coli, methicillin resistant Staphylococcus aureus, metallo-β lactamase-producing Pseudomonas aeruginosa and extended spectrum β-lactamase-producing Klebsiella pneumoniae. The LABs were grown on tryptic soy agar (TSA) medium and overlaid with TSA inoculated with the MDR test bacteria. Ten LAB isolates were identified using 16S rDNA sequencing. All were identified as Lactobacillus rhamnosus. The results also showed that all the LAB isolates were negative for antibacterial activities.