Histological study of the influence of vitamin E on the development of Anas sp. heart and liver

• Main Authors: Cerda, Christlyn Marol C., Rey, Cathrina Marie B.
• Format: text
• Language: English
• Published: Animo Repository 2012
• Subjects: Vitamin E; Anas—Spermatozoa—Morphology; Biology; Poultry or Avian Science
• Online Access: https://animorepository.dlsu.edu.ph/etd_bachelors/5302
• Institution: De La Salle University

This study was done to determine the morphological effects of varying doses of Vitamin E, an antioxidant, on the heart and liver of duck eggs (Anas sp.) by descriptive analyses of their histological sections. Sixty fertilized duck eggs were used in the study: The eggs were divided into three groups and were injected in ovo on the fifth day of incubation. The first group served as a Control group. The Normal Dose Group received 0.25 mL of Vitamin E, the normal dosage of vitamin E in ratio of average infant weight to average duck eggs collected. The Double Dose Group received 0.50 mL of vitamin E, the double dose of the Normal Dose group. On the twenty-seventh day of incubation, the eggs were all cracked open and 5 ducklings were randomly dissected from each group. The heart and liver were removed and processed for histological analyses. A photomicroscope was used for the examination and documentation of the slides. The results showed that there were no significant adverse histological change on the heart and liver when compared to the control groups. This indicates that normal doses and double doses of vitamin E do not affect its (vitamin E) metabolism by the liver that in turn does not affect heart processes. The histological results may be explained by the inaccurate computation of the dosage given to the ducks disregarding the active form of the vitamin, also, the vitamin E used was probably not well absorbed by the duck egg giving a negative result.