Isolation of acid phosphatase from cassava (Manihot Esculenta Crantz syn M. Utilissima Pohl) and kamote (Ipomea Bataas)

Isolation of acid phosphatase from cassava (Manihot Esculenta Crantz syn M. Utilissima Pohl) and kamote (Ipomea Bataas)

Acid Phosphatase was isolated and purified from cassava (Manihot Esculenta Crantz syn M. utilissima Pohl) and Kamote (Ipomea batatas) by ammonium sulfate precipitation, centrifugation, and dialysis. The protein concentration was determined through Biuret's test. The activity of the enzyme was m...

Full description

Saved in:
Bibliographic Details
Main Authors: Hernandez, Lawrence Nemaiah A., Pimenta, Leslie Elline N.
Format: text
Language:English
Published: Animo Repository 2002
Subjects:
Cassava
Ipomoea
Chemistry
Online Access:https://animorepository.dlsu.edu.ph/etd_bachelors/5760
Tags: Add Tag
No Tags, Be the first to tag this record!
Institution: De La Salle University
Language: English
id oai:animorepository.dlsu.edu.ph:etd_bachelors-6404
record_format eprints
spelling oai:animorepository.dlsu.edu.ph:etd_bachelors-64042021-07-15T08:10:18Z Isolation of acid phosphatase from cassava (Manihot Esculenta Crantz syn M. Utilissima Pohl) and kamote (Ipomea Bataas) Hernandez, Lawrence Nemaiah A. Pimenta, Leslie Elline N. Acid Phosphatase was isolated and purified from cassava (Manihot Esculenta Crantz syn M. utilissima Pohl) and Kamote (Ipomea batatas) by ammonium sulfate precipitation, centrifugation, and dialysis. The protein concentration was determined through Biuret's test. The activity of the enzyme was monitored using the conversion of p-nitrophenyl phosphate to p-nitrophenol by acid phosphatase. The colored product of the reaction was determined spectrophotometrically. High Performance Liquid Chromatography was used to confirm the presence of acid phosphatase. 2002-01-01T08:00:00Z text https://animorepository.dlsu.edu.ph/etd_bachelors/5760 Bachelor's Theses English Animo Repository Cassava Ipomoea Chemistry
institution De La Salle University
building De La Salle University Library
continent Asia
country Philippines
Philippines
content_provider De La Salle University Library
collection DLSU Institutional Repository
language English
topic Cassava
Ipomoea
Chemistry
spellingShingle Cassava
Ipomoea
Chemistry
Hernandez, Lawrence Nemaiah A.
Pimenta, Leslie Elline N.
Isolation of acid phosphatase from cassava (Manihot Esculenta Crantz syn M. Utilissima Pohl) and kamote (Ipomea Bataas)
description Acid Phosphatase was isolated and purified from cassava (Manihot Esculenta Crantz syn M. utilissima Pohl) and Kamote (Ipomea batatas) by ammonium sulfate precipitation, centrifugation, and dialysis. The protein concentration was determined through Biuret's test. The activity of the enzyme was monitored using the conversion of p-nitrophenyl phosphate to p-nitrophenol by acid phosphatase. The colored product of the reaction was determined spectrophotometrically. High Performance Liquid Chromatography was used to confirm the presence of acid phosphatase.
format text
author Hernandez, Lawrence Nemaiah A.
Pimenta, Leslie Elline N.
author_facet Hernandez, Lawrence Nemaiah A.
Pimenta, Leslie Elline N.
author_sort Hernandez, Lawrence Nemaiah A.
title Isolation of acid phosphatase from cassava (Manihot Esculenta Crantz syn M. Utilissima Pohl) and kamote (Ipomea Bataas)
title_short Isolation of acid phosphatase from cassava (Manihot Esculenta Crantz syn M. Utilissima Pohl) and kamote (Ipomea Bataas)
title_full Isolation of acid phosphatase from cassava (Manihot Esculenta Crantz syn M. Utilissima Pohl) and kamote (Ipomea Bataas)
title_fullStr Isolation of acid phosphatase from cassava (Manihot Esculenta Crantz syn M. Utilissima Pohl) and kamote (Ipomea Bataas)
title_full_unstemmed Isolation of acid phosphatase from cassava (Manihot Esculenta Crantz syn M. Utilissima Pohl) and kamote (Ipomea Bataas)
title_sort isolation of acid phosphatase from cassava (manihot esculenta crantz syn m. utilissima pohl) and kamote (ipomea bataas)
publisher Animo Repository
publishDate 2002
url https://animorepository.dlsu.edu.ph/etd_bachelors/5760
_version_ 1712576482615754752

Similar Items