Isolation and antibacterial screening of actinomycetes from soil and leaf samples collected within the DLSU campus

Isolation and antibacterial screening of actinomycetes from soil and leaf samples collected within the DLSU campus

A total of forty isolates of actinomycetes were obtained from soil and leaf samples within the DLSU campus. Of theses, 25 came from leaves and 15 came from soil. Isolation used the technique prescribed by Tunac (1993) with Glycerol Yeast Extract Agar (GYEA) as culture medium. Isolates were described...

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Bibliographic Details
Main Authors: Villanueva, Walter L., Hernandez, Alet Gerardo C.
Format: text
Language:English
Published: Animo Repository 1995
Subjects:
Actinomycetales
Leaves > Microbiology
De La Salle University
Soil micro-organisms
Antibacterial agents
Micro-organisms, Effect of antibiotics on
xx4 Soils > Bacteriology
Online Access:https://animorepository.dlsu.edu.ph/etd_bachelors/6144
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Institution: De La Salle University
Language: English
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Summary:A total of forty isolates of actinomycetes were obtained from soil and leaf samples within the DLSU campus. Of theses, 25 came from leaves and 15 came from soil. Isolation used the technique prescribed by Tunac (1993) with Glycerol Yeast Extract Agar (GYEA) as culture medium. Isolates were described in terms of cultural characteristics. Screening for antibacterial properties of the isolates was done using the Cross Streak method. Of the 25 isolates from leaves, 72 percent (18) inhibited Bacillus subtilis, 56 percent (14) inhibited Staphylococcus aureus, and 24 percent (6) inhibited Pseudomonas aeruginosa. Of the 15 isolates from the soil, 47 percent (7) inhibited S. aureus, 40 percent (6) inhibited B. subtilis, and 27 percent (4) inhibited P. aerugenosa. Of the 40 isolates, 60 percent (24) inhibited P. aeruginosa, 53 percent (21) inhibited S. aureus and 25 percent (10) inhibited P. aerugenosa.

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