Antimicrobial flavonoid from Hibiscus rosa-sinensis Linn

• Main Author: Rufino, Leslie Ann A.
• Format: text
• Language: English
• Published: Animo Repository 2006
• Subjects: Malvaceae; Antimicrobial agents; Anti-infective agents; Chemistry
• Online Access: https://animorepository.dlsu.edu.ph/etd_masteral/3561; https://animorepository.dlsu.edu.ph/context/etd_masteral/article/10399/viewcontent/CDTG004348_P.pdf; https://animorepository.dlsu.edu.ph/context/etd_masteral/article/10399/filename/0/type/additional/viewcontent/CDTG004348_F.pdf
• Institution: De La Salle University

Hibiscus rosa-sinensis afforded 5,7,4-trihydroxyflavanone (Hr-1), and a mixture of hydrocarbons and squalene (Hr14). The structure of Hr-1 was elucidated by extensive 1D and 2D NMR spectroscopy, while the presence of squalene was deduced by comparison of its 1H-NMR spectral data with those reported in the literature. Hr-1 was tested for antimicrobial potential by the agar well method against seven microorganisms. It indicated low antibacterial activity against Staphylococcus aureus, and low antifungal activity against Candida albicans and Trichophyton mentagrophytes. Hr-1 was tested for cytotoxicity using a human lung non-small cell adenocarcinoma (A549) cell line and a representative of other normal cell types, normal chinese hamster ovarian (AA8) cell line. Results of the study indicated that Hr-1, has no linear interpolation with the A549 cell line, thus IC50 could not be computed and Hr-1 is inactive.